Protein Synthesis -Translation and Regulation
what is the role of DNA in protein synthesis
DNA-driven nanoparticle assembly provides a powerful tool for the fabrication of superlattices with tunable composition, structure, and inter-particle distance. DNA is chemically stable and can be conveniently applied to functionalize nano-objects and provide a high degree of encoding in the assembly process. The high specificity of DNA—its unique ability to recognize and bond with complementary sequences—permits the programming of nanoparticle interconnections and allows us to create multi-functional materials. We can then leverage that synthesis knowledge to investigate any emergent and collective phenomena.
what role does mRNA play in protein synthesis
For example, RNA has three major subtypes: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three of those subtypes are involved in protein synthesis.
Ribosomes and Protein Synthesis
The mutant drpA1 defines a new gene in Escherichia coli K-12 that maps at about 5.2 min. This mutation was obtained after enriching a population of cells for temperature sensitive dna mutations with the [3H]thymidine 'suicide' technique followed by screening for mutants defective in transposon Tn5 precise excision. When growing cells carrying the drpA1 allele were shifted to the nonpermissive temperature, we showed that DNA, RNA, and protein synthesis shut off quickly, with the cessation of RNA synthesis occurring first. A recombinant plasmid between pBR322 and an HindIII fragment from wild-type E. coli restores the growth defect in drpA1 mutants. Using transposon Tn5 mutagenesis of this plasmid, we have been able to correlate the presence of a 68-kilodalton protein, as observed with the maxicell technique, with the ability of this plasmid to restore growth to drpA1 mutants.