Wolbachia mediators of inflammation and their role in …

coli MetC has a small promiscuous alanine racemisation activity, but no detectable glutamate racemisation activity.Two of the clades —the genus Pelagibacter and the family Anaplasmataceae, which includes Wolbachia— were in the same class, the Alphaproteobacteria, but the precise location was under debate in the literature.

The interaction of Wolbachia bacteria with eukaryotic cells

The investigation of obligate intracellular Wolbachia endobacteria is extremely challenging.

Interactions between the inherited bacterium Wolbachia …

Dobson says he expects approval in a few months, which would let mosquito-control districts in the US add the method to their arsenal against Aedes albopictus. As for the Wolbachia-infected Aedes aegypti in Clovis, MosquitoMate has sought permits for additional tests in California and Florida. If those go well, MosquitoMate will ask the EPA to approve its mosquitoes as a pesticide as well.

Co-evolution between parthenogenesis-inducing Wolbachia and ..

Community support will be key as the EPA considers MosquitoMate’s applications for the use of Wolbachia-infected mosquitoes. The application has received 10 comments, at least three of them from people who confused the Wolbachia mosquitoes with genetically modified insects. For example: “I’m 100 percent against this plan to release GMO mosquitos in FL.”

japonica have Wolbachia-infected parthenogenetic populations and uninfected sexual populations.

through field release of wolbachia-infected mosquitoes and ..

In conclusion, intracellular Wolbachia are able to replicate outside their host cell and further steps for the establishment of an extracellular culture system as a powerful tool for molecular biological approaches are discussed.

"Molecular Mechanisms Defining the Effect of Host Diet …

Many species of arthropod are infected by matrilineally inherited bacteria. One of these bacteria, Wolbachia, has received much attention because it is common and also has a variety of interactions with its host. Wolbachia may therefore be an important driver of host evolution and ecology. However, the dynamics of Wolbachia-host interactions are poorly understood, except in theoretical terms. In this thesis, I demonstrate that the presence of a male-killing strain of Wolbachia in the butterfly Hypolimnas bolina has selected for a host modifier that suppresses the male- killing action in Southeast Asian populations. I further demonstrate that suppression of male-killing reveals the Wolbachia to have a second phenotype, namely induction of cytoplasmic incompatibility. This new phenotype explains the persistence of the infection following the evolution of male-killer suppression. Mathematical simulations of the dynamics of suppression indicate that novel suppressors spread rapidly - agreeing with historical data that suggest the presence of male-killing in Southeast Asia in the recent past. Ancient H. bolina DNA is utilised to gain further information about historical processes in this system. Flux in the Wolbachia-H. bolina interaction is demonstrated - with the infection rising and falling in prevalence in different host populations. A search for a third phenotype, a direct benefit, provides no clear evidence of the existence of such an effect in this system. The thesis concludes with a general discussion in which it is argued that Wolbachia-bost interactions may be more dynamic than previously recognised, with strong selection driving the spread of new mutations through the host population, modifying the phenotype produced by the bacterium. The system has in the past experienced high sex ratio bias due to the presence of male-killing Wolbachia, with concomitant effects on host ecology. My thesis predicts this phenotype will become largely lost in the next 100 years from this species.

I molecular cloned the T4SS coupling protein VirD4 from wPip and analyzed the co-transcription between T4SS and Wolbachia surface protein in wStr.

Mathematical and Natural Sciences

Elsewhere in the world, . And entomologists with the project are taking advantage of another quirk of Wolbachia: its ability to prevent mosquitoes from passing the Zika, dengue, and yellow fever viruses onto humans. The downside is the number of mosquitoes remains unchanged, so they keep biting and biting. The upside is that this method is cheaper; a one-time release of female mosquitoes can infect an entire population with Wolbachia, whereas males must be continuously released.

Third, different ages of the PI-Wolbachia infection may have different consequences for the host species.

Wolbachia Biology > Epidemiology of Microbial …

In this thesis, I studied the causes, consequences and dynamics of a parthenogenesis-inducing (PI) Wolbachia infection in two hosts, the parasitoid wasps Tetrastichus coeruleus (Hymenoptera: Eulophidae) and Asobara japonica (Hymenoptera: Braconidae).

First, different scenarios may occur for the spread of PI-Wolbachia infection in different host species.

Molecular studies of Wolbachia and sex-determination …

(2007) Interactions between the inherited bacterium Wolbachia and the butterfly Hypolimnas bolina. Doctoral thesis , University of London.