Central Dogma and Protein Synthesis
("The Central Dogma" of Protein Synthesis, as ..
The binding of specific tRNAs to the ribosome is generally facilitated by the sequence of the mRNA, but this protein manages to bypass what was once perceived as a crucial step in the protein-making process. This results in a nonsensical tag of alanines and threonines added to the end of improperly made proteins.
One potential purpose of this tag may be to target the dysfunctional protein for degradation. Accumulation of degenerate proteins has been linked to neurodegenerative diseases, such as Alzheimer’s and Huntington’s. The study authors suggest this new method of protein synthesis could contribute to disease prevention.
Effective hooks regarding protein synthesis and the central dogma.
Peter Shen, Ph.D., and co-authors first arrived at this interesting finding when they used cryo-electron microscopy to determine the structure of the quality control proteins bound to a stalled ribosome. They observed that Rqc2p was bound to both the ribosome and a tRNA, which it had positioned adjacent to the improperly made protein chain. Seeing is not always believing, though, so the scientists involved in the study carried out numerous biochemical assays to validate this new method of protein synthesis.