Cycle of Photosynthesis and Respiration - VTAide

The Grossman Lab has identified many mutants in Chlamydomonas defective in photosynthetic function. A streamlined platform is being developed to analyze these mutants which involves spectroscopy, fluorometry, CO2 fixation and oxygen evolution. Some of the basic parameters for the putative regulatory mutants that we will monitor include: (1) Growth on minimal medium; (2) Light sensitivity, which will be performed on cells grown on plates exposed to light intensities ranging from 0-150 μmol photon m-2s-1 (as shown in Fig. 4); (3) Pigment analysis, from whole cell spectra and spectra of pigments extracted in 80% acetone (and potentially some HPLC analysis of pigments), from which we will calculate levels of chlorophylls, carotenoids and the chlorophyll a/b ratio (104); (4) Gas exchange measurements, including both O2 evolution (in the light) and uptake (in the dark), using a Clark-type O2 electrode. These experiments coupled with the use of specific inhibitors could yield information on whole chain electron transport and the partial reactions of photosynthesis (e.g. PSII, inter-chain carriers, PSI), and potentially provide some insights into alternate electron flow. Many measurements of other photosynthetic parameters will be facilitated by the use of the Dual PAM 100 fluorometer, the fast repetition rate pulse fluorometer, and the JTS10 spectrophotometer (see Equipment & Facilities). The spectrophotometry and fluorescence analyses are rapid, have high information content and are noninvasive, making them ideal for a primary mutant screen. Many of the specific activities measured in these assays are described below.

Easy Science for Kids Photosynthesis: How Plants Make Food and Energy

How does this investigation demonstrate that plants give off oxygen during  photosynthesis?

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The light-independant reactions of photosynthesis occur in the stroma of the chloroplast and involve the conversion of carbon dioxide and other compounds into glucose. The light-independent reactions can be split into three stages, these are carbon fixation, the reduction reactions and finally the regeneration of ribulose bisphosphate. Collectively these stages are known as the Calvin Cycle.

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Variables: The variables that might affect the rate of photosynthesis in this experiment are: Temperature: When the temperature rises so does the rate of photosynthesis; this is because as the temperature around the plant rises the enzymes controlling photosynthesis inside the chloroplasts heat up and start moving around faster, the fast moving molecules collide with other fast moving enzymes causing them to react....

Photosynthesis is important because animals eat food, as it is a source of energy.

ICARP2014 - Artificial Photosynthesis

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The Plan In my experiment I am going to see how light affects the rate of photosynthesis.

SCIENCE AND TECHNOLOGY LOG: PHOTOSYNTHESIS - YouTube

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Aim To see whether light effects the rate of Photosynthesis in plants or not....

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Through reading various books and web pages it was made clear that carbon dioxide is definitely one of the raw materials needed for photosynthesis, but I wanted to see whether this is actually true and if carbon dioxide is taken away completely will the plant photosynthesize at all.

Photosynthesis mainly takes place in the palisade mesophyll cell in the leaves of plants.

photosynthesis notes - Biology Junction

As we can see, there is a close relationship between the action spectrum and absorption spectrum of photosynthesis. There are many different types of photosynthetic pigments which will absorb light best at different wavelengths. However the most abundant photosynthetic pigment in plants is chlorophyll and therefore the rate of photosynthesis will be the greatest at wavelengths of light best absorbed by chlorophyll (400nm-525nm corresponding to violet-blue light). Very little light is absorbed by chlorophyll at wavelengths of light between 525nm and 625 (green-yellow light) so the rate of photosynthesis will be the least within this range. However, there are other pigments that are able to absorb green-yellow light such as carotene. Even though these are present in small amounts they allow a low rate of photosynthesis to occur at wavelengths of light that chlorophyll cannot absorb.