Cycle of Photosynthesis and Respiration - VTAide
The Grossman Lab has identified many mutants in Chlamydomonas defective in photosynthetic function. A streamlined platform is being developed to analyze these mutants which involves spectroscopy, fluorometry, CO2 fixation and oxygen evolution. Some of the basic parameters for the putative regulatory mutants that we will monitor include: (1) Growth on minimal medium; (2) Light sensitivity, which will be performed on cells grown on plates exposed to light intensities ranging from 0-150 μmol photon m-2s-1 (as shown in Fig. 4); (3) Pigment analysis, from whole cell spectra and spectra of pigments extracted in 80% acetone (and potentially some HPLC analysis of pigments), from which we will calculate levels of chlorophylls, carotenoids and the chlorophyll a/b ratio (104); (4) Gas exchange measurements, including both O2 evolution (in the light) and uptake (in the dark), using a Clark-type O2 electrode. These experiments coupled with the use of specific inhibitors could yield information on whole chain electron transport and the partial reactions of photosynthesis (e.g. PSII, inter-chain carriers, PSI), and potentially provide some insights into alternate electron flow. Many measurements of other photosynthetic parameters will be facilitated by the use of the Dual PAM 100 fluorometer, the fast repetition rate pulse fluorometer, and the JTS10 spectrophotometer (see Equipment & Facilities). The spectrophotometry and fluorescence analyses are rapid, have high information content and are noninvasive, making them ideal for a primary mutant screen. Many of the specific activities measured in these assays are described below.
Easy Science for Kids Photosynthesis: How Plants Make Food and Energy
Artificial photosynthesis: The two-in-one technology …
The light-independant reactions of photosynthesis occur in the stroma of the chloroplast and involve the conversion of carbon dioxide and other compounds into glucose. The light-independent reactions can be split into three stages, these are carbon fixation, the reduction reactions and finally the regeneration of ribulose bisphosphate. Collectively these stages are known as the Calvin Cycle.
solar-fuels generation Science and technology
Variables: The variables that might affect the rate of photosynthesis in this experiment are: Temperature: When the temperature rises so does the rate of photosynthesis; this is because as the temperature around the plant rises the enzymes controlling photosynthesis inside the chloroplasts heat up and start moving around faster, the fast moving molecules collide with other fast moving enzymes causing them to react....
ICARP2014 - Artificial Photosynthesis
Chatbots, computer programs designed to simulate conversations with human users, are gaining momentum in business. Many industries, including the property sector, are beginning to use Chatbots for customer service interactions. By appearing more real and human than automated phone calls, ‘bots’ are being welcomed by the public over automated phone calls. Like most AI technology, Chatbots can help property agents save on time and money.