A Novel Synthesis Process for Making Nickel Oxide Nanoparticles
Optimization of Nickel Oxide Nanoparticles Synthesis through ..
The study of new biosynthetic routes of metal oxide NPs is important to identify alternatives to chemical and physical methods. After biosorption, a fraction of the dead H. lixii biomass loaded with NPs was examined by TEM and HRTEM (Figs and ). The TEM and HRTEM images provided information about the location of the nickel oxide NPs in the dead biomass and revealed the presence of extra and intracelular NPs. shows absence of NPs in the control. Ultrastructural changes included the shrinking of cytoplasmatic material in the control and in the biomass containing the metal NPs, which was probably the result of the autoclaving process (). TEM analysis revealed a predominantly spherical shape of the extra and intracellular nickel oxide NPs, which had an average diameter of 3.8 and 1.25 nm, respectively. The NPs formed inside the fungal cells were smaller than extracellularly produced NPs. The extra and intracellular synthesis of NPs of other metals has been reported, but those studies have used live fungal biomass .
Synthesis of nickel oxide nanoparticles using pulsed …
In this study only dead biomass of H. lixii was used for the analysis of nickel oxide NPs production since it exhibited a high adsorption capacity of the nickel metal ion than live and dried biomass. Biosynthesis of nickel oxide NPs by dead biomass of H. lixii was investigated under the following conditions: contact time of 90 min, initial pH 4.0, temperature of 30°C, agitation speed of 150 rpm, 1.0 g of the biosorbent, and a solution containing 100 mg L-1 of nickel (II). The synthesized nickel oxide NPs were characterized by transmission electron microscopy (TEM) (JEOL-1010) operating at 100 kV, high-resolution transmission electron microscopy (HRTEM) (JEOL JEM2100 equipped with a LaB6 gun) operating at 200 kV, X-ray photoelectron spectroscopy (XPS) (UNI-SPECS UHV System), scanning electron microscopy (SEM) (JEOL 6460 LV) equipped with an energy dispersive spectrometer (EDS) and infrared vibrational spectroscopy FTIR (Bruker ALPHA) using an attenuated total reflectance accessory of single reflection (ATR with platinum-crystal diamond).