Synthesis of prostaglandins and leukotrienes

Although a pregnancy-related increase in neutrophil and monocyte counts may explain these observations, granulocytes of pregnant donors have lower leukotriene-synthetic capacities.

Increased Synthesis of Leukotrienes in the Mouse …

What is the fate of exogenous prostaglandin endoperoxide, PGH2, in isolated lung (Abstract)?

Gender variation in the synthesis of leukotrienes.

Leukotrienes are eicosanoids which means they are a collection of oxygen derivatives of some 20 different essential fatty acids. The other eicosanoids are prostaglandins, prostacyclins and thromboxanes. Leukotrienes are produced along with the release of histamine. Leuko trienes are one class of eicosanoids lipid mediators which means they are signalling molecuels which occur by the of essential fatty acids at the site of inflammation or where is required. These are derived from omega-3 fatty acids or the omega-6 fatty acids. There is no storage for leukotrienes as they have to be synthesized wherever they are required.

Total Synthesis of Leukotrienes from Butadiene, …

White Blood cells or WBC’s are known for their immunity building role in our body. Leukotrienes are one of the defense weapons of white blood cells which are lipids and play a pivotal role in immunity increasing function. They can also be expressed as the hormones which cause symptoms in asthma and hay (allergic rhinitis). They are responsible for activating many different types of white cells so as to fight the foreign material/organism invasion in our body. Biologists have found out that malfunctioning of Leukotrienes can result in aggravation of asthma and other diseases too.


American Journal of Respiratory and Critical Care Medicine

Although cells of the myeloid lineage are well-recognized sources of leukotriene production, an alternative synthetic pathway for leukotrienes involves transcellular metabolism., In this two-cell process of leukotriene synthesis, the donor cell (believed to be a bone marrow-derived cell, such as the neutrophil or the macrophage) generates LTA4, which then passes transcellularly to the recipient cell, where it is further metabolized to downstream leukotrienes, depending on the enzymatic repertoire of the recipient cell. In a model of neuronal ischemia in the brain, glial cells demonstrated the enzymatic capability to produce leukotrienes by transcellular biosynthesis. It is hypothesized that the local recipient cell generation of leukotrienes may cause focal areas of prolonged inflammation and tissue injury.,,

Prostaglandins, Leukotrienes & Essential Fatty Acids

The basal and stimulated synthesis of immunoassayable 12- and 5-monohydroxyeicosatetraenoic acids (HETE) and leukotrienes (LT) B4 and C4 was studied in glomeruli isolated from rats with nephrotoxic serum glomerulonephritis (NSGN) induced by low (30 micrograms/g body weight) or high (105 micrograms/g) doses of anti-rat glomerular basement membrane (GBM) immunoglobulin (Ig). In the early heterologous phase of the disease, low doses of anti-GBM Ig enhanced the basal synthesis of 12-HETE but not that of 5-HETE or LT. High anti-GBM Ig doses enhanced the basal synthesis of 5-HETE and LTB4 as well. Under stimulated conditions, enhanced glomerular production of 5-HETE and LTB4 occurred at 15 min after infusion of anti-GBM Ig, peaked at 1 h, and returned toward control levels by 24 h. At 48 h, 72 h, and on day 12, the synthesis of these eicosanoids was impaired. Neutrophile depletion only partially reduced glomerular eicosanoid synthesis after induction of NSGN whereas complement depletion significantly reduced 5-HETE, 12-HETE, and LTB4. These observations indicate that in the heterologous phase of NSGN there is enhanced but short-lived glomerular 5-HETE and LTB4 synthesis. This phenomenon is mediated by complement activation and may be an important proinflammatory event leading to capillary wall injury in the early stages of the disease.

Prostaglandins and Leukotrienes: Advances in …

Various enzymes catalyze different chemical reactions in a series to enable the generation of Leukotrienes from arachidonic acid through a typical type of white cells – Leukocytes. Also it has been found that some times other intermediate products are used by different types of cells from the leukotriene pathway to convert them into thriving Leukotrienes biologically.

The Leukotrienes - 1st Edition - Elsevier

Mouse retinas or cultured retinal cells were sonicated in RIPA buffer (25 mM Tris, pH 7.4, 1 mM EDTA, 150 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% deoxycholic acid, 1 mM phenylmethylsulfonyl fluoride, 1 μg/mL leupeptin, and 1 μg/mL aprotinin). Cultured retinal glial cells and mRECs were homogenized in buffer containing protease inhibitors (leupeptin, 1 μg/mL; aprotinin, 1 μg/mL; 1 mM, PMSF; 0.2 mM, Na3VO4). Protein content of retinal and cultured cell samples was quantified (Bio-Rad, Hercules, CA). Equivalent amounts of sample proteins were loaded, separated by SDS-PAGE, and transferred to nitrocellulose membranes. After overnight blocking in 5% nonfat dry milk, the blots were probed with primary antibodies for leukotriene B4 receptors (BLT1 and BLT2) and the species-specific secondary antibody. After extensive washing, protein bands were visualized by enhanced chemiluminescence and evaluated by densitometry. Membranes then were stripped and reprobed with antibody against tubulin to confirm equal protein loading. Densitometric analysis was performed using the public domain NIH image program developed at the National Institutes of Health with the Scion Image 1.63 program.