Synthesis of chemically modified DNA

Glen Research is delighted to introduce a GalNAc modification strategy using a monomeric GalNAc support and the equivalent GalNAc phosphoramidite. Our experimental work has shown that these products are fully compatible with regular oligonucleotide synthesis and deprotection. Oligonucleotides containing GalNAc can be deprotected using standard procedures during which the acetyl protecting groups on the GalNAc group are removed. Glen Research offers these GalNAc C3 products under an agreement with AM Chemicals LLC.

Microarray analysis using disiloxyl 70mer oligonucleotides

* = The yield guarantee is calculated for unmodified oligonucleotides (20mers).

OligoCalc: an online oligonucleotide properties calculator

New oligonucleotide analogues with triazole internucleotide linkages were synthesized, and their hybridization properties were studied. The analogues demonstrated DNA binding affinities similar to those of unmodified oligonucleotides. The modification was shown to protect the oligonucleotides from nuclease hydrolysis. The modified oligonucleotides were tested as PCR primers. Modifications remote from the 3′-terminus were tolerated by polymerases. Our results suggest that these new oligonucleotide analogues are among the most promising triazole DNA mimics characterized to date.

Synthesis of chemically modified DNA

The chemical synthesis of DNA oligonucleotides (DNA Primers) has a long tradition at BioTeZ. For 25 years, we are dealing with technology to synthesize DNA sequences. As a research-intensive company, we have a fine grasp on our client’s requirements concerning oligos / primer in order to make experiments and applications successful and efficient.

Induced cross-linking reactions to target genes using modified oligonucleotides

RNA Oligonucleotides - Integrated DNA Technologies

Every DNA base (in terms of DNA synthesis chemistry, we are speaking of phosphoramidite monomers and amidites) added during DNA synthesis has a dimethoxy-trityl (trityl) protecting group attached to the 5´-hydroxyl position. This acid labile trityl-group is bound to the 5’-end of each support-bound monomer and protects the corresponding base from undergoing unwanted chemical reactions during the synthesis cycle. The trityl-group is removed in the first step of each synthesis cycle, immediately before a new base is added, until the elongation of the nucleotide chain is complete. The final trityl-group is removed before delivery (Unless otherwise requested).

Formation of N-branched oligonucleotides as by-products in solid-phase oligonucleotide synthesis

Tib Molbiol - Oligonucleotides, Design for PCR and …

For oligonucleotides ordering or enquiries, you can use the order form on this website or just send your order / enquiry by email to

Synthetic oligonucleotides are single stranded DNA fragments assembled from single mononucleosides, following a defined and specific sequence.

DNA/RNA Oligonucleotides - Microsynth - CH

Synthetic oligonucleotide purification is particularly challenging because of the small differences in size, charge and hydrophobicity between the full-length product and impurities, which often co-elute.

Turnaround time for all standard orders is usually 24 hours, i.e. oligonucleotides are available on the next business day.

Synthesis and use of synthetic oligonucleotides.

The trityl-group is colorless when attached to a DNA base but it gives a characteristic orange color once removed. The intensity of this color can be measured by UV spectrophotometry and it is directly related to the number of trityl molecules present. Following the first coupling step, the amount of trityl released during deblocking is directly proportional to the amount of full-length oligo synthesized in the previous cycle. When the trityl is cleaved during the deblocking step, the resulting trityl cation is orange in color. The intensity of this color can be measure by UV spectrophotometry. By comparing the intensities of the trityl cation produced after the first and last coupling steps, one can calculate the average successful base coupling per cycle and hence the coupling efficiency.