Microarray analysis using disiloxyl 70mer oligonucleotides
OligoCalc: an online oligonucleotide properties calculator
New oligonucleotide analogues with triazole internucleotide linkages were synthesized, and their hybridization properties were studied. The analogues demonstrated DNA binding affinities similar to those of unmodified oligonucleotides. The modification was shown to protect the oligonucleotides from nuclease hydrolysis. The modified oligonucleotides were tested as PCR primers. Modifications remote from the 3′-terminus were tolerated by polymerases. Our results suggest that these new oligonucleotide analogues are among the most promising triazole DNA mimics characterized to date.
Synthesis of chemically modified DNA
The chemical synthesis of DNA oligonucleotides (DNA Primers) has a long tradition at BioTeZ. For 25 years, we are dealing with technology to synthesize DNA sequences. As a research-intensive company, we have a fine grasp on our client’s requirements concerning oligos / primer in order to make experiments and applications successful and efficient.
RNA Oligonucleotides - Integrated DNA Technologies
Every DNA base (in terms of DNA synthesis chemistry, we are speaking of phosphoramidite monomers and amidites) added during DNA synthesis has a dimethoxy-trityl (trityl) protecting group attached to the 5´-hydroxyl position. This acid labile trityl-group is bound to the 5’-end of each support-bound monomer and protects the corresponding base from undergoing unwanted chemical reactions during the synthesis cycle. The trityl-group is removed in the first step of each synthesis cycle, immediately before a new base is added, until the elongation of the nucleotide chain is complete. The final trityl-group is removed before delivery (Unless otherwise requested).