What Is the Role of DNA in Protein Synthesis? (with pictures)

Since immunoblotting studies indicated differentialexpression of both XIAP and survivin protein in mesothelioma celllines () we furtherinvestigated the role of these proteins in resistance to cisplatinusing RNAi mediated knockdown. Previously, in our laboratory(unpublished data) we observed that mRNA knockdown by RNAi may notcorrelate with protein. Therefore, we employed cell based ELISAassays for XIAP and survivin to optimise for RNAi mediatedknockdown at the protein level.

What Is the Role of RNA in Protein Synthesis

This lesson discusses the role of RNA in protein synthesis

03/11/2013 · Role of RNA in Protein Synthesis

In doing so, siRNA prevent the production of specific proteins based on the nucleotide sequences of their corresponding mRNA. The process is called RNA interference (RNAi), and may also be referred to as siRNA silencing or siRNA knockdown.

What Is the Role of DNA in Protein Synthesis

Small interfering RNA (siRNA) are small pieces of double-stranded (ds) RNA, usually about 21 nucleotides long, with 3' overhangs (2 nucleotides) at each end that can be used to "interfere" with the translation of proteins by binding to and promoting the degradation of messenger RNA (mRNA) at specific sequences.

Read about the role of Argonaute proteins in the RNAi ..
To learn more about RNAi's role in protein synthesis, check out RNAi Explained.

What is the role of messenger RNA and ribosomes in protein synthesis

The central role of RNA in protein synthesis is illustrated by the fact that many antibiotics used to fight infections bind to the ribosomal RNA of bacteria and block the production of cellular protein. This prevents the bacteria from growing. Errors in the production or sequence of the RNA components of the protein synthesis machinery can also cause disease in humans, including, Diamond Blackfan anemia, caused by a defect in the production of ribosome, Dyskeratosis congenita, caused by a defect in ribosomal RNA structure, and some forms of diabetes, myopathies and encephalopathies due to mutations in transfer RNA.

A mechanism for the control of protein synthesis by adenovirus VA RNAI

Repress protein synthesis and regulate development of organism.

The regulation of eIF4E's activity by 4E-BPs (TOR substrates) could provide a mechanism by which TOR controls longevity. However, no structural ortholog of the 4E-BPs has been identified in C. elegans although it is possible that functional orthologs do exist. However, knocking down TOR extends lifespan further in ife-2 deleted worms, indicating that the effects of TOR and ife-2 are mediated through distinct mechanisms [], further discussed in section 4.2. Thus, TOR's effects on lifespan in the worm model appear to operate by additional mechanisms. For example, TOR signaling promotes ribosome biogenesis, a costly process that is needed for cell growth and division, across the eukaryota from yeast to mammals []. Since the number of ribosomes in a cell governs the cellular capacity for protein synthesis, decreased ribosome production may be involved in extending lifespan in animals where TOR signaling is downregulated.

RNAi Phenotypes and the Localization of a Protein::GUS Fusion Imply a Role ..

The role of Myc-induced protein synthesis ..

The deliberate use of RNAi by plant scientists to reduce gene expression in plants (now usually called gene 'knockdown') has become common in recent years. Single-stranded that hybridized to a complementary, single-stranded, sense mRNA was deliberately introduced into plant cells to achieve knockdown. While plant biologists first believed that the resulting dsRNA helix could not be translated into a protein, it is now clear that the dsRNA triggered a RNAi response. The experimental use of dsRNA in biological research became more widespread after the discovery of the RNAi machinery, first in petunias and later in .