Gene Expressionand Protein Synthesis

Primers of genes selected for qRT-PCR; protein abundance and localization of identified proteins in 2-day-old soybean; changed nuclear proteins at 51 h compared to 48 h after sowing; changed nuclear protein under flooding stress for 3 h compared to 48 h after sowing; changed nuclear proteins identified in control and flooding stress with time course manner; identification of protein synthesis related proteins from total protein fraction in soybean under flooding stress for 24 h; procedures of nuclei enrichment; pictures of Western blot analysis for histone H3; principal component analysis of proteomic data; mapping of nuclear pore complex related proteins using KEGG pathway database; cluster analysis of significantly changed nuclear proteins in root tip of soybean in the initial stages of flooding stress; functional distribution of significantly changed nuclear proteins in the identified clusters; agarose gel electrophoresis of qRT-PCR products ()

The journey from gene to protein is ..

How do genes direct the production of proteins

Role of DNA in Protein Synthesis - Learn Online at CCC

The sequence of the nucleotides in DNA can be referred to as the genetic code.Each portion of the nucleotide sequence in DNA that is responsible for the length andsequence of amino acids in one particular protein can be called a gene.Our DNA seems to be ultimately responsible for the size, shape and function of everyprotein that our bodies make. Similarly, the DNA of each plant and animal seems to beultimately responsible for the size, shape and function of every protein that each of themmakes.

Gene Function: Protein Synthesis Flashcards | Quizlet

by Lynn MarieWartski

AAAS Benchmark

Protein synthesis, the process of genes contributing to a phenotype, is complex and can be difficult for students to learn. Watching their teacher demonstrate their understanding of it while they teach is not enough for the students to truly understand what is going on at the cellular and molecular levels. They need to practice using these ideas, making mistakes and learning from them, in an environment with some support, before they are expected to use these concepts on their own when encountering new phenomena. This is a great activity to allow students to do just that as they work with a model of protein synthesis that involves their entire classroom. The teacher's desk is the nucleus containing the DNA sequences. In teams of 3, student 1 becomes the mRNA when they transcribe the DNA in nucleus and return to student 2, the ribosome. The ribosome (student 2) writes out the needed tRNA sequence to match the mRNA. Student 3, tRNA, looks for the correct sequence in the cytoplasm (classroom) and when they find it they flip up the card to reveal a word (amino acid). They return to the ribosome with their word (amino acid) to build a sentence (protein). The number of sentences you have your students work through would depend on when it takes them to get comfortable with this process before they move on to examine different types of mutations.

at the ribosomal site of protein synthesis during ..

After identifying the reading frame in a DNA sequence, you determine the amino acid sequence and then try to create each of the mutations described at this website. Notice the impact each mutation has on the protein product and imagine the impact it could have on the phenotype for that trait. This is an event that results in genetic variation for that trait. Not all mutations will have the same impact on the trait and its phenotype. Developed by The Genetic Science Learning Center at the University of Utah. ( - AAAS Benchmarks and - )

Gene Expression and Protein Synthesis

by Lisa Weise, Lori Buwalda, and Barb Neureither

AAAS Benchmark

When teaching protein synthesis, you may want to use this activity if you go into more detail. Include the ideas that when the DNA is transcribed into RNA it must then have the sections that represent the introns removed leaving only the exons which makeup the mRNA(click here for more and ). As the human genome project progressed, scientists realized we don't have 100,000 genes as was originally thought. The new estimate became 75,000, then 50,000, then 35,000, and now around 25,000 (it may have changed since this was written). If that is true, then the idea that one gene makes one protein seems impossible since we have more proteins than genes. So how is this possible? Scientists now believe that a single gene can make multiple proteins by splicing together different combinations of their exons. That is what this activity is all about. Using the idea from the previous activity, Protein Synthesis and Words, they make their DNA sequence result in a group of words that can make more than one sentence (protein). By spicing out the introns (nonsense words) and combining different groups of exons (words) they end up with different proteins (sentences).

What is the role of mRNA in protein synthesis? - Updated …

: Brush up on your understanding of heredity, DNA, genes, traits, chromosomes and proteins at this interactive website developed by The Genetic Science Learning Center at the University of Utah.

: Review your understanding of the structure of DNA and how it replicates as you build a DNA molecule at this interactive website developed by The Genetic Science Learning Center at the University of Utah.

Ribosomes and Protein Synthesis | Biology I

In this activity, we will use the three letter words in a sentence to model the effect of different mutations on a sequence of DNA and its resulting protein. All mutations are not alike; therefore the resulting proteins and phenotypes may differ due to genetic variation (multiple alleles) for that single gene. ( - AAAS Benchmarks and - National Science Content Standard )