A protonmotive force drives ATP synthesis in bacteria.Proc.
Protons consumed per ATP synthesized
From the thermodynamicconsiderations less then 3 protons pro ATP is hardly feasible, for theenergy required for ATP synthesis under physiological conditions isabout 50 kJ mol-1(~520meV), so atphysiological values inthe range of 120-200 mV at least 3 protons should be transferred to getthe energy necessary.
10/4 (because 4 protons per ATP) --> 2.5 ATP.
4) Nice movies at
From the early experiments with mitochondria the H+/ATPratio for ATP synthesis was estimated as 3.
ATP Synthase Flashcards | Quizlet
Two protons transferred per ATP synthesised after flash activation of chromatophores from photosynthetic bacteria.FEBS Lett.97:367–372
what part of ATP synthesis catalyzes ATP hydrolysis
A comparison between the phosphate potential and the electrochemical proton gradient showed that the data found are in agreement with a stoichiometry of 2 protons translocated per molecule ATP synthesized.
Hydrolysis and Synthesis of ATP by Membrane-Bound …
The dominant strategy involves a molecule called ATP, which is synthesized from ADP and phosphate through the enzymatic action of a special protein (ATP synthase). Naturally, since this process is essential for the functioning of any organism, ATP synthase has been major research subjects for many years. An analysis of the enzyme structure by Masasuke Yoshida led to a prediction by Paul Boyer that it actually rotates while converting ADP to ATP and storing energy. Rotation is not the only significant aspect, since it was also predicted that the catalysis functions like a motor with two driving units and a common shaft. This view was furthered by J. E. Walker through an X-ray crystallographic analysis. Still, firm evidence was needed. This was recently achieved by Yoshida, who attached a very long (1-3 micrometers) actin filament with a fluorescent marker to the upper motor (10 nanometer diameter) and actually made a video of the rotation.