Drag-and-Drop Protein Synthesis: Quiz - zeroBio
Initiation of Protein Synthesis
Sep 5, 1988. The trmD operon of Escherichia coli encodes the ribosomal proteins S16 and L19, the tRNAm1G37methyltransferase and a 21,000 Mr protein. TRNA, and that the synthesis of ribosomal protein, like the synthesis of rRNA, is subject to the influence of the rel gene control system. In exponentially growing.
what is initiation, elongation, and termination
Ribosome feedback regulation, and growth rate-dependent controls of rRNA synthesis remain to be determined despite numerous investigations. r-protein. REGULATION OF RIBOSOMAL PROTEIN SyNTHESIS. control of ribosomal synthesis can be considered in relation to three basic problems 75.
Initiation of Protein Synthesis in Bacteria
N2 - The mechanisms of action of cycloheximide and emetine in exponentially growing Chinese hamster ovary cells have been studied (a) by measuring the incorporation of [35S]methionine into N-terminal and internal positions of nascent peptide chains by an Edman degradation modified for handling a large number of samples and (b) by determining the size distribution of polysomes in the presence of the drugs. These combined techniques have confirmed that all three phases of protein synthesis (initiation, elongation, and termination) are susceptible to inhibition by cycloheximide and have shown that the primary sensitive step varies with the concentration of drug between 10-9 and 10-3 m. At the lowest doses, initiation appears, by these criteria, to be the step most sensitive to inhibition by cycloheximide, while emetine seems to act primarily on elongation.
The site of protein synthesis is the ribosome
In addition to the mRNA template, many molecules and macromolecules contribute to the process of translation. The composition of each component may vary across species; for instance, ribosomes may consist of different numbers of rRNAs and polypeptides depending on the organism. However, the general structures and functions of the protein synthesis machinery are comparable from bacteria to human cells. Translation requires the input of an mRNA template, ribosomes, tRNAs, and various enzymatic factors.