synthesis and growth in Octopus vulgaris.

Synthesis and application of research: All the knowledge and practical experience I gained during my STSM will be applied during my PhD project at the Stazione Zoologica in Naples: I am planning to use extracellular recording to study sensory perception and nociception in Octopus vulgaris, in order to investigate the physiological features of the fibers running along the axial nerve cord of the arm and to identify any high-threshold responding unit, or units responding only to chemical noxious stimuli.

synthesis and growth in Octopus vulgaris

Growth and food intake models in Octopus vulgaris ..

Growth and Food Intake Models in Octopus Vulgaris ..

Serin protease inhibitor (SERPIN) proteins are important elements of the host defense to inactivate proteases secreted by pathogens and restrict their invasion , . Protease inhibitors have been found in Crassostrea virginica, C. gigas, Chlamys farreri and Ruditapes philippinarum, but have not been described in cephalopods. A total of 6 transcripts corresponding to SERPIN were putatively identified in the O. vulgaris library. Biochemical, functional and molecular characterization of SERPIN is needed to understand whether and how the octopus’ hemocytes use this protein to counteract coccidiosis.

Growth and food intake models in Octopus vulgaris Cuvier ..

Antimicrobial peptides are proteins with the broad ability to kill or neutralize Gram-negative and Gram-positive bacteria, fungi, parasites or viruses, interacting with and crossing cell envelope membranes by a multihit mechanism . The bactericidal permeability-increasing protein (BPI) is an AMP produced by polymorphonuclear leukocytes, but also by epithelial cells. Mucosal epithelia that co-exist with microbes and microbial products expressing BPI probably contribute to the maintenance of immunologic homeostasis at mucosal surfaces . At least three light-organ proteins in the BPI/LBP (lipopolysaccharide-binding protein) family have been sequenced from E. scolopes. A single transcript of the BPI protein is provided in this O. vulgaris library. Additional studies are needed to understand the role of this protein in the octopus cellular defense.

Protein synthesis and growth in Octopus vulgaris ..
Abstract— The process of protein synthesis in the brain of Octopus vulgaris ..

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With high-throughput Illumina Solexa sequencing technology, we have generated 59,859 high quality sequences from 12,918,391 paired-end reads. Using BLASTx/BLASTn, 12,227 contigs have blast hits in the Swissprot, NR protein database and NT nucleotide database with E-value cutoff 1e−5. The comparison between the Octopus vulgaris central nervous system (CNS) library and the Aplysia californica/Lymnaea stagnalis CNS ESTs library yielded 5.93%/13.45% of O. vulgaris sequences with significant matches (1e−5) using BLASTn/tBLASTx. Meanwhile the hit percentage of the recently published Schistocerca gregaria, Tilapia or Hirudo medicinalis CNS library to the O. vulgaris CNS library is 21.03%–46.19%. We constructed the Phylogenetic tree using two genes related to CNS function, Synaptotagmin-7 and Synaptophysin. Lastly, we demonstrated that O. vulgaris may have a vertebrate-like Blood-Brain Barrier based on bioinformatic analysis.

A synthetic gene encoding the Octopus vulgaris protein sequence was ..

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Summary of research completed during STSM: During my short term scientific mission on the CIIMAR Interdisciplinary Center of Marine and Environmental Research of the University of Porto supervised by Dr. Filipe Castro allowed me increasing my knowledge related to the identification and analysis of enzymes involved to lipid metabolisms. Especially about polyunsaturated fatty acids (PUFAs), and the role of DHA, as well as a high proportion of phosphatidylcholine (PC), due to are essential for a good growth and development of the paralarvae. The main bottleneck of rearing Octopus vulgaris paralarvae occurs during the planktonic stage and is caused by several factors, among which nutritional deficiencies are believed to play a key role. The arm growth is a key factor for the change from a planktonic to benthonic life strategy or what is the same, to the next stage: juveniles. In paralarvae, the development of the arm also comprises protein and neuronal synthesis. The genesis of neurons during development is significantly influenced by the proportion of uridine, choline, and the omega-3 fatty acids docosahexaenoic acid (DHA, EPA and ALA) and phosphatidylcholine (PC) in the diet. The final reaction of the Kennedy pathway is the condensation of CDP-choline with diacylglycerol (DAG from DHA) to form Ptd-Cho in a reaction catalyzed by CDP-choline: 1,2-diacylglycerol choline phosphotransferase (CPT).

01/12/2007 · Abstract. Gestal, C., Guerra, A., and Pascual, S. 2007. Aggregata octopiana (Protista: Apicomplexa): a dangerous pathogen during commercial Octopus vulgaris …

The Adaptive Response of Protein Turnover to the …

Next Generation Sequencing libraries for Octopus vulgaris were obtained from the SRA. A total of two libraries from 454 platforms and two from Illumina were downloaded and de novo assembled using Newbler 2.8 and Velvet 1.2.10 software respectively. Contigs generated from the de novo assembly were blasted (Blastx) against the annotated proteomes from Zebrafish (Danio rerio) and Fruitfly (Drosophyla melanogaster) as a vertebrates and invertebrate models respectively, using BioEdit free software. Data mining from the annotated transcriptome was used to identify genes of interest. The list of genes retrieved was defined by literature search and were included stress related genes, metabolism related genes, protein synthesis and degradation and muscle growth related genes. Sequences retrieved were cured to only contain the coding regions, eliminating UTR regions and miss-assembly regions. Data mining was also done in the O. bimaculoides genome in order to complete possible holes in the pathways and systems analysed. The degree of similarity between O. vulgaris and O. bimaculoides was established by blasting (Blastn) the de novo transcriptomes against the O. bimaculoides predicted genes from the EnsemblMetazoa database using BioEdit. In addition, maximum likelihood phylogenetic trees were constructed to test if vertebrate annotation could be valid to apply to octopus orthologues.