examples of cDNA synthesis and cloning;
Problems with cDNA gene amplification | Biocompare: …
Using Invitrogen's Superscript III First strand synthesis Supermix and followed by Accuprime Supermix PFX for cDNA amplification.
I've been trying to get my RT-PCR to work but I keep on having very very little to no bands when I run the cDNA product on an agarose gel. My starting RNA is harvested from mouse liver using the Qiagen kit and I used oligo dts. Not even the + control HeLa included in the superscript kit is giving me a strong band. I started using Extaq from Takara with no success so I switched to Accuprime Supermix but still no good. I checked the cDNA primers with a mouse liver panel as a positive control and they came out great. What am i doing wrong?
28/08/2015 · Problems with cDNA gene amplification
I have seen many gel photos of first-strand cDNA synthesis (including my own), but the results seem to range from heavy smears without visible rRNA bands, to faint smears with bright rRNA bands.
Several posts in these forums have already touched upon this question (for example