Cell wall defective variant of Nocardia rubra.
Plant cell wall: structure and biosynthesis | HSTalks
Drakakaki G, Park E, Worden N. Probing post-Golgi trafficking and polysaccharide deposition with chemical genomics and proteomics. Plant Cell Walls, Gordon Research Conference, Colby College. August 5-10, 2012. Poster.
Fourth Conference on Biosynthesis of Plant Cell Wall
Drakakaki G. “Dissection of endomembrane trafficking and cell wall deposition” University of Vermont, USA. April 4, 2013. Invited talk.
Plant Cell Wall Biosynthesis, S
To test the effect of heterologous expression of PtrMYB152 on potential target genes, we examined the expression of a suite of secondary cell wall biosynthetic genes in Arabidopsis PtrMYB152 expressing lines that exhibited altered cell wall properties. The analyses were carried out using seedlings, pooled from three independent lines for each construct, to ensure tissue of uniform age for each genotype. Using seeding rather the stems also allowed us to identify genes that may be directly activated by PtrMYB152. As shown in , the transgenic lines had increased expression of several lignin biosynthetic genes including 4CL1 (At1g51680), HCT (At5g48930), C3H1 (At2g40890), CCoAOMT1 (4g34050) and CAD6 (Atg37970). This suggests that PtrMYB152 regulate secondary cell wall biosynthesis by activating a subset of secondary cell wall biosynthetic genes.
Cell Walls and Plant Growth/Cell Expansion, D
As shown in , total lignin content in the transgenic plants was elevated, with an 24% and 18% increase for 35:PtrMYB152 and PtrCesA8:PtrMYB152 relative to wild-type respectively. We further examined the relative amounts of syringyl (S) and guaiacyl (G) monomers in the inflorescence stem cell walls, which showed that the S/G monomer ratio was higher in the transgenic plants than wild-type plants (). The significantly increased interfascicular fiber cells wall thicknesses of the transgenic lines may explain this alteration, since such cells develop the bulk of secondary walls in the Arabidopsis inflorescence stem, and fiber cells are enriched in S-lignin.