Biology Experiment, Photosynthesis | Photosynthesis | …



If placed in a suitable nutrient environment, cells and tissues of many organisms are ableto reproduce and form new plants or animals. Now, we will deal with vegetable tissues,whose culture is simpler than that of animal cellules and tissues. It is necessary toprepare a nutritive and sterilized culture medium for the piece of plant tissue. Keep theculture in the suitable conditions of light and temperature and which vary from plant toplant. Over many days, you will observe the growth of a callus or roots or shoots. In thisway you can obtain even whole plants (cloning). These experiments show that special cellskeep all the information necessary to generate the whole plant.
As we have mentioned, it is necessary avoid bacteria and moulds in the cultures. For thisyou will need sterilize tools, vials, tubes, and nutrient medium. Place each in anautoclave for a ten minutes or, lacking an autoclave, a pressure cooker. The tissues aswell have to be free from microorganisms and they have to be sterilized with bleach (40%solution for 15 min) or with alcohol.
The transfer of the tissues into the test tubes has to be made in aseptic conditions,using a sterile box. Lacking that, make your first trials in a quiet place, as devoid ofwind and dust as possible. The culture medium should contain water, vitamins (particularlythose of the B-complex. For this, use yeast extract), sugars, mineral salts. To enrich thewater with mineral salts, boil some water with a handful of soil, then let settle andfilter it. Usually, people also insert 0.5-0.8% of agar-agar to "solidify" themedium. As culture medium, coconut milk has been used. It contains mineral salts, sugars,vitamins and growth hormones.
1 - For yours first tests of micropropagation, use strawberries tissues.
2 - If this simple experiment interests you, you can continue on the way of the invitro culture of vegetable tissues. In fact you can propagate a lot of plants in thisway. Plants easy to culture are the following: tomato, potato, strawberry, chrysanthemum,geranium, sunflower, tobacco, carrot and onion. You can use tissues obtained from seeds,such as the embryo, but you can use also tissues taken from adult plants, such as tissuesof roots, stems, apical buds, shoots, leaves, even single cells. Each plant and tissue hasits own needs. They are different from each other. You can try the influence of thevegetable hormones, special nutrients, etc.
This field is very broad and complex so, if you are interested in continuing with theseexperiments, you can buy special books and you should build a sterile box.
Plant Tissue Culture for the Gardener
Basic Principle in Plant Tissue Culture Technique
Plant Tissue Culture Kit Manual
Plant Micropropagation Using African Violet Leaves
Plant Tissue Culture (links)
Internet keywords: in vitro culture plant tissue micropropagation.

Biology Paper 2 | Photosynthesis | Experiment - Scribd

For this experiment, you will need a can/bin made of plastic, wood, or metal with a removable lid.

IB Biology: Photosynthesis Experiments

The soil is composed of many different sized particles. With this simple experiment you can separate the main components of the soil and evaluate their proportions.
1 - Go into a field an collect a sample of soil. Put it in a jar of water. Stir it well and let it settle. Observe and describe the different layers of materials.
2 - In water, particles settle more quickly the bigger they are. It is possible to use this property to determine the amount of each component of the soil. Put 3 parts water and 1 part of soil in the container (try 1 cup soil and three cups of water in a quart jar); shake the container for 5 minutes and let the material set. With reference to the figure 1, after 40 seconds measure the thickness of sediment. Call this A; after 30 minutes measure again and call this B; after 24 hours measure C. Now, by subtraction, you can determine the thickness of the main layers: C-B = layer of clay, B-A = layer of silt, A = layer of gravel and sand. Using a sieve with 2 mm holes (less than 1/8 inches), you can separate the gravel from the sand and determine their ratio. On the basis of these data, calculate the content (%) of each component of the soil sample.
3 - Repeat the same experiment with soil collected in other places or that have a different geological origin (i.e: meadow, wood, river bank) or anyplace the soil has a different consistency or texture (i.e: muddy, sandy). Describe the composition of each soil and try to explain the differences. You can also apply this technique to evaluate the composition of the soil for a potted plant, and correct it. Example: if water doesn't drain well, would more sand help? If it needs to hold water longer would clay or organic matter be helpful?
4 - With a microscope, measure the size of the particles. With a clock, measure the time to drop to the bottom of a jar of water. time of the particles in water as a verses their size. Then graph with the Y-axis for the size of the particle and X-axis the time to fall.
Soil and Environment Activities
Soil description and classification
Internet keywords: soil sedimentation test.

Biology Experiment How To Prove : Photosynthesis …



The bottle garden is a close relative of the biosphere: a tiny garden growing inside abottle, a glass sphere, or a demijohn. The main difference is that soil is the maincomponent not water. The container can be kept sealed or opened like a terrarium. Ourswill be more for just plants whereas the terrarium may have animals. These gardens in abottle, can be cured and perfected as a system, they are also very decorative, in fact youcan treat them as a bonsai with not only a single tree, but whole landscapes. A carefullychosen stone becomes a rock, a small plot of moss represents a great prairie. A contorted,little plant is now a hundred year old tree. Maybe a marsh wetlands with peat moss,mushrooms and ferns is more to your liking. Maybe appropriate succulent plants with sandand rocks for a vast desert landscape. The choice is yours. There is no rigid rule aboutbeing either an open or a closed garden. It maybe useful to keep the lid on to retainwater. Or eventually, the plants grow up and need to hang down the outside.
If the container is always sealed, the challenge becomes quite demanding. In fact, it thenis necessary to obtain an equilibrium between plants and animals in order to keep aneffective cycle of substances and energy, but if this is difficult in a predominantlywater environment, it is even more difficult in a just moist environment. So, the gardenhas to contain a moist soil, plants, little animals like terrestrial isopods (i.e.: sowbug, pill bug), earthworms, etc. Plants have to be resistant to high moisture and have asmall size and limited growth. To avoid roots rotten, you can also try to refer tohydroponics and substitute the ground with small sized expanded clay or with gravel. Inthis case you will have more difficulties in hosting animal species, so it will have to beleft open. It is important you place your garden in a cool and well illuminated place, butaway from direct sunlight. Open or closed, a bottle garden is a fascinating thing, and theidea itself of a little and self-sufficient world is fascinating as well. There are peopleso fond of bottle gardens they work at them a long time, taking care of them using longand thin tools and to observe these environments till they imagine to be one of theirinhabitants.
Then choose among these main options:
1 - Opened bottle garden, containing moist ground, plants and possible little animals. Itis suited for people who love taking care of gardens and who have a flair for
the architecture of little wide spaces.
2 - Opened bottle garden, prepared in hydroponics and containing plants. It is suited forthose who tend to forget to water their plants.
3 - Sealed bottle garden, holding moist soil, plants, and some little animals. Thisexercise is suitable for people who are keen on biology and scientific research. They willhave to find appropriate plants and animals suitable for a sealed ecosystem and will haveto achieve the necessary conditions for a long survival. This research, bibliographic inpart and experimental, will keep students happily busy for long time.
The educational value of these little ecosystems is evident. They can be carried out evenin a school, where the teacher will be allowed to illustrate their characteristics topupils and encourage interesting discussions. In the web sites below, you will findimportant practical information in building your bottle gardens.
Terrariums : Miniature Worlds in a Bottle!
BBC online, how to gardening, bottle garden
Forum
Un jardin en bouteille
Le terrarium : Un monde miniature dans une bouteille!
Internet keywords: garden bottle jar, jardin bouteille bonbonne.

You can come up with many such biology experiment ideas, if you are a keen observer of nature.
The results from our experiment showed that the amount of CO2 in the environment of the spinach had an effect on the rate of photosynthesis.

Read on for some biology experiment ideas



Let us see now how protists and other little animals of ponds react to alteration to theirenvironment.
1 - Some microscopic algae, like the euglena, search out light (phototaxis) and to do thisthey use an organelle sensible to the light, named stigma. With a dark paper, cover thebottom part of a test tube holding a culture of euglena. The part of the test tube exposedto light should become green, rich with algae. Make the same experiment with othermicroscopic algae and with protozoa.
2 - Add two or three drop of distilled water to a little water drop collected in a pondand watch what happens to the protists. Very probably you will see them inflate and thenexplode. This occurs because of the different saline concentration inside and outside theprotists and the osmotic pressure which is produced inside their cells.
3 - Protists are sensitive to most chemicals and generally they react by running away; insome cases instead they approach them (chemotaxis). Prepare some microscope slides withprotists and observe through the microscope their behavior when you add acidic substances(i.e.: vinegar), basic substances (i.e.: backing soda), glucose, salt, sparkling water(rich of CO2), broth, milk, tiny grain of cheese, dyes, etc. At thebeginning use very low amounts of these substances, then increase their concentration.
4 - From a pond or an aquarium, collect a hydra and place it on a microscope slide with apair of water drops. Observing this tiny polyp through the microscope, probably you willsee some sucker shaped microorganisms (trichodina) moving on its body. Watch what happensafter adding a little drop of vinegar to their water! Trichodina will escape from thehydra and probably die. Hydra itself will have launched many of its harmful paralyzingdarts.
5 - Submit protists to different stimulus such as light, temperature, electric field(about 5 V in DC). In this last case, some protists will gather on the cathode (thenegative - pole). Also amebas are inclined to move towards the cathode. Change thepolarity of the current and observe the behavior of the protists.
Internet Keyword: phototaxis chemotaxis protists.

This portion was used to get acquainted with experimental procedure and to investigate the many variables that control photosynthesis.

This question paper consist of two section: Section A and Section B


After a shower, the soil is soft and the animals which walked there, leave tracks. Bymeans of plaster, you can obtain the mould of these tracks and make an
interesting collection (figure 9). The experiment is very simple: it is a question ofbringing a little plaster in dry form, some water, a bowl to mix the plaster,
and a spoon to stir. But, to which animal belong the tracks you have picked up? From herestart the research of information. They exist books which give the picture of the tracksof many animals. Moreover, recognizing the animal which left the track is not sufficient:it is necessary to know something of its behavior, etc. From the simple collection oftracks you will be curious to know the animals of your land. Our article: "" provides you some indication on this topic. This experiment will openyour eyes so you can observe the territory around you when you walk in naturalenvironments with more attention. Plaster can be used also to obtain the mould of fruits,barks and other natural objects. From the cast you can also obtain the original shape.

Class practical In this experiment the rate of photosynthesis is measured by counting the number of bubbles rising from the cut end of a piece of Elodea or Cabomba.

Photosynthesis: Biology Lab - Video & Lesson …

So the hypothesis that water is the nutrient used by plants was rejected.

Repeat John Woodward's experiment:




In August of 1771, , an English Chemist, put a sprig of mint into a transparent closed space with a candle that burned out the air (oxygen was not discovered yet) until it soon went out.