Oligo Design and Analysis Tools - IDT

Methods for reliable synthesis of long genes offer great promise for novel protein synthesis via expression of synthetic genes. Current technologies use computational methods for design of short oligos, which can then be reliably synthesized and assembled into the desired target gene. A precursor to this process is optimization of the gene sequence for improved protein expression. In this thesis, we provide the first results on the computational complexity of oligo design for gene synthesis. For collision-oblivious oligo design -- when mishybridizations between oligos are ignored -- we give an efficient dynamic programming algorithm. We show that an abstraction of the collision-aware oligo design problem is NP-hard. We extend our collision-oblivious algorithm to achieve collision-aware oligo design, when the gene can be partitioned into independently-assembled segments. We propose additional algorithms for gene optimization and demonstrate their utility for collision-aware design. All methods are evaluated on a large biological gene set.

Custom Oligo Synthesis - General Biosystems

Includes online.FL cDNA clones: Searchable collection of over 33,000 non-redundant, Gene Synthesis.

Oligo Analysis Tool - Eurofins Genomics

The BLOCK-iT™ RNAi Designer is such an effective tool for the design of Stealth RNAi™ siRNA Cloning Gene Synthesis.
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Oligo Design & More; Oligo Analysis Tool .

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Oligo DNA synthesis
Oligo DNAsynthesis

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Oligo DNA synthesis

Oligo Design & More; Primer Design Tools

Protocol for Real-Time RT-PCR Author.
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gRNA Synthesis Protocol ( such as pCR-Blunt II-TOPO from Invitrogen.
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Guideline to reference gene selection for (iii) differences in RNA preparation and cDNA synthesis, since the reference gene is exposed to (Invitrogen.
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DNA & RNA Synthesis; General Oligo Use; Oligo Purification; ..