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Conclusions-The hypertriglyceridemic effect of Angpt14 is attributable to inhibition of LPL-dependent VLDL lipolysis by converting LPL dimers to monomers, and Angpt14 upregulates cholesterol synthesis in liver secondary to inhibition of LPL-and HL-dependent hepatic cholesterol uptake.

Fatty Acid Synthesis | Lipoprotein | Triglyceride

Lipoprotein lipase (LPL, red) is synthesized primarily by skeletal muscle and adipose tissue

01/01/1999 · Basic Med Biochem.I

Methods and Results-In 24-hour fasted mice, Angpt14 overexpression increased plasma triglycerides (TG) by 24-fold, which was attributable to elevated VLDL-, IDL/ LDL-and HDL-TG content. Angpt14 overexpression decreased post-heparin LPL activity by stimulating conversion of endothelial-bound LPL dimers to circulating LPL monomers. In fasted but not fed state, Angpt14 overexpression severely impaired LPL-dependent plasma TG and cholesteryl ester clearance and subsequent uptake of fatty acids and cholesterol into tissues. Consequently, hepatic cholesterol content was significantly decreased, leading to universal upregulation of cholesterol and fatty acid synthesis pathways and increased rate of cholesterol synthesis.

Association Mapping and Marker Development of …

Triglycerides made up oflong chain fatty acids, in the form of chylomicrons (from intestinalabsorption) or lipoproteins (from hepatic synthesis), are hydrolyzedto glycerol and free fatty acids by an enzyme called lipoproteinlipase (LPL).

LPL synthesis (adipocytes) ..
17/10/2007 · Angptl4 Upregulates Cholesterol Synthesis in Liver via Inhibition of LPL- and HL-Dependent Hepatic Cholesterol Uptake

Dr. Namrata Chhabra | Biochemistry for Medics – Lecture …

AB - Fatty acid ethyl esters (FAEEs), nonoxidative by-products of ethanol metabolism, are found in various tissues and plasma after ethanol ingestion and may be responsible for some of the pathological changes observed in alcohol-consuming individuals. Previous studies demonstrated that several different enzymes, including lipoprotein lipase (LPL), can catalyze FAEE synthesis in vitro. We report that LPL cetalyzes FAEE synthesis in isolated rat hearts perfused with chylomicrons in the presence of ethanol. Most of the FAEEs accumulated in the perfusate, suggesting that in vivo, plasma FAEEs derive from LPL-mediated synthesis. Our results are the first demonstration of the direct involvement of a specific enzyme, LPL, in FAEE synthesis under physiological conditions.

However, there are limited data concerning the action of LPL on the regulation of milk fat synthesis in goat mammary gland.

High Cholesterol, APOE Gene and Diet - GB HealthWatch

N2 - Gram-negative sepsis suppresses lipoprotein lipase (LPL) activity in adipose tissue which contributes, in part, to the altered clearance of triglycerides. The suppression in LPL activity occurs when plasma insulin concentrations are elevated and insulin-stimulated glucose utilization is impaired. This study was planned to evaluate whether the presence of insulin resistance was responsible for the decrease in adipose LPL activity. Adipocytes were isolated from epididymal fat pads 24 h after inducing sepsis in male Lewis rats by intravenous injection of 4 x 108 colonies of live Escherichia coli/100 g body wt. The decrease in heparin-releasable (HR) LPL activity in adipocytes from the septic rats was evident at the time of isolation and maintained in a 20-h culture. After overnight incubation with insulin (10-8 M), HR LPL activity was stimulated to a greater extent in adipocytes from septic rats (298%) than in adipocytes from control rats (88%). The insulin stimulation of LPL activity during sepsis could not be attributed to insulin-like growth factor-I (IGF-I) as adipocytes from septic rats appeared to be IGF-I resistant. Insulin-treatment (10-8 M) increased LPL synthesis 99% in adipocytes from control rats and 136% in adipocytes from septic rats. Insulin treatment also led to a 65 and 62% increase in LPL mass in adipocytes from control and septic rats, respectively. These findings indicate that the sepsis-induced decrease in adipose LPL is not due to insulin resistance with respect to LPL. The insulin stimulation of LPL activity in adipocytes from septic rats appears to be mediated by an increase in LPL synthesis.

The data indicate that IFN-gamma is inhibiting macrophage LPL at least in part via a reduction of LPL synthesis. Paper-6540600.

Low-fat diets can help reduce the risk for these diseases

Abstract: Lipoprotein lipase (LPL) serves as a central factor in hydrolysis of triacylglycerol and uptake of free fatty acids from the plasma. However, there are limited data concerning the action of LPL on the regulation of milk fat synthesis in goat mammary gland. In this investigation, we describe the cloning and sequencing of the LPL gene from Xinong Saanen dairy goat mammary gland, along with a study of its phylogenetic relationships. Sequence analysis showed that goat LPL shares similarities with other species including sheep, bovine, human and mouse. LPL mRNA expression in various tissues determined by RT-qPCR revealed the highest expression in white adipose tissue, with lower expression in heart, lung, spleen, rumen, small intestine, mammary gland, and kidney. Expression was almost undetectable in liver and muscle. The expression profiles of LPL gene in mammary gland at early, peak, mid, late lactation, and the dry period were also measured. Compared with the dry period, LPL mRNA expression was markedly greater at early lactation. However,