Long, Processive Enzymatic DNA Synthesis Using 100% …
But synthesising long DNA sequences ..
The real answer lies in the resolution limit of the purification method and on the coupling efficiency of the DNA synthesizer. We can synthesize DNA oligos of 220 bases and obtain sufficient quantities by purification to perform successful gene construction. However, it should be remembered that the longer the oligo, the greater the chance of accumulated sequence errors.
Long DNA strands synthesis optimizing - ResearchGate
All of the DNA synthesis reagents have an impact on good quality DNA synthesis, as are all the steps in the synthesis cycle – coupling, capping, oxidation and detritylation. However, for the successful synthesis of long oligos, some factors are absolutely critical, as noted below.
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The long, precisely labeled DNA duplexes obtained behave in very much the same manner as natural DNA and are beyond the range of chemical synthesis. Moreover, the conditions of synthesis closely resemble the natural ones, and all the artifacts accompanying the chemical synthesis of DNA are thus eliminated. The approach proposed seems to be completely general and could be used to label DNA at multiple pre-determined sites and with halogen derivatives of any nucleobase. Access to DNAs labeled with Hal-NBs at specific position is an indispensable condition for the understanding and optimization of DNA photo- and radio-degradation, which are prerequisites for clinical trials of Hal-NBs in anticancer therapy.