iScript cDNA Synthesis Kit | Life Science Research | Bio …

In general 1 ng to 1 μg total RNA or 0.1-100 ng mRNA arerecommended.

First Strand cDNA SynthesisReaction
Denaturation of RNA and primer at 70°C for 5 minutes canremove secondary structures that may impede long cDNA synthesis.

iScript cDNA Synthesis Kit | | Bio-Rad

The kit alsocontains two optimized primers for reverse transcription and nuclease-freewater.
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Order Bio-Rad's iScript™ cDNA Synthesis Kit on ZAGENO

For example, 5minutes incubation is enough for a 2 kb cDNA synthesis.

Choice ofPrimers for Reverse Transcription
Oligo d(T) priming is preferredfor most applications because it ensures that all cDNA copies terminate at the3´ end of the mRNA and produces the longest contiguous cDNA.

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The first strand cDNA productgenerated is more than 10 kb (Figure 1).

General Information forSuccessful cDNA Synthesis:

Template RNA
Intact RNA of high purity is essential for sensitive RT-PCR detection.

The iScript™ cDNA synthesis kit performs across a broad range of concentrations
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ProtoScript® First Strand cDNA Synthesis Kit | NEB

UltraScript Reverse Transcriptase is a robust and thermostable reverse transcriptase engineered to enhance cDNA synthesis speed and yield with accurate transcript representation. The latest developments in reverse transcriptase technology and buffer chemistry allow for efficient, non-biased and sensitive cDNA synthesis.

Poisoning cDNA kit (iScript), exceeding linear range?

The PrimeScript First Strand cDNA Synthesis Kit contains all of the reagents necessary for synthesis of first-strand cDNA from total or poly(A)+ RNA using PrimeScript Reverse Transcriptase. Choose this cDNA synthesis kit when you want to synthesize full-length cDNA with provided primers (select from oligo dT or random 6-mer primers, both included in the kit.) This cDNA synthesis kit is powered by PrimeScript Reverse Transcriptase, which has exceptionally strong strand displacement activity and efficiently synthesizes cDNA. PrimeScript RTase is robust, versatile and well-suited for applications requiring full-length cDNA such as cDNA library construction. This PrimeScript-based cDNA synthesis kit also works well with other cDNA-dependent techniques including RT-PCR, cDNA probe preparation and real-time quantitative RT-PCR (qRT-PCR or RT-qPCR). The PrimeScript First Strand cDNA Synthesis Kit is ideal for reverse transcription of many different RNA templates, including GC-rich templates and RNAs with high levels of secondary structure.

TATAA GrandScript cDNA Synthesis Kit - tataa

First introduced in Japan, PrimeScript is now available to scientists worldwide. Over 3,700 peer-reviewed articles have been published in which PrimeScript Reverse Transcriptase was referenced in a variety of scientific applications such as gene expression, gene discovery, transcriptome analysis, miRNA expression and regulation, molecular evolution, virology and microbiology. PrimeScript RTase is a modified, recombinant MMLV (Moloney Murine Leukemia Virus) reverse transcriptase and is verified to have low levels of RNase H. Because of the excellent extension capability of PrimeScript RTase, cDNA synthesis reactions can be performed at a lower temperature (42°C), decreasing the risk of RNA degradation that can occur during conventional reactions performed at higher temperatures.

cDNA synthesis/reverse transcription kit review - Labome

Random Primer Mixoffers good performance in a wide range of RNA templates.

When agene-specific primer is used in a cDNA synthesis reaction, the cDNA product canbe used only for amplification of that transcript.