Here are the results:Analysis of the synthetic gene.

Synthetic DNA oligonucleotides are assembled in order with subsequent strand closure employing LCR (Ligase Chain Reaction) and/or other PCR techniques.

oligonucleotide; gene synthesis; nucleic acids; synthetic biology

Oligonucleotide synthesis - Wikipedia

Oligonucleotide synthesis is the chemical synthesis of relatively ..

Microarray synthesis of DNA oligonucleotides costs a fraction of a penny per base. But such methods can’t make pieces longer than about 200 bases. And stitching those oligonucleotide fragments into gene-length sequences typically must be done one strand at a time, losing the multiplex advantage of the array synthesis.

Artificial gene synthesis - Wikipedia

And stitching those oligonucleotide fragments into gene-length sequences typically must be done one strand at a time, losing the multiplex advantage of the array synthesis.

recombinantplasmid DNA (recDNA), genomic DNA, RT-PCR product, commerciallysynthesized big oligonucleotide.

custom oligo synthesis, oligonucleotides ..

2004 Jul 1;32(Web Server issue):W176-80

Gene2Oligo is a program that divides a long sequence into a set of contiguous oligonucleotides corresponding to both DNA strands.

Antisense Oligonucleotides - Integrated DNA …

Conjugation of small interfering RNA (siRNA) to an asialoglycoprotein receptor ligand derived from -acetylgalactosamine (GalNAc) facilitates targeted delivery of the siRNA to hepatocytes and . The ligands derived from GalNAc are compatible with solid-phase oligonucleotide synthesis and deprotection conditions, with synthesis yields comparable to those of standard oligonucleotides. Subcutaneous (SC) administration of siRNA–GalNAc conjugates resulted in robust RNAi-mediated gene silencing in liver. Refinement of the siRNA chemistry achieved a 5-fold improvement in efficacy over the parent design with a median effective dose (ED50) of 1 mg/kg following a single dose. This enabled the SC administration of siRNA–GalNAc conjugates at therapeutically relevant doses and, importantly, at dose volumes of ≤1 mL. Chronic weekly dosing resulted in sustained dose-dependent gene silencing for over 9 months with no adverse effects in rodents. The optimally chemically modified siRNA–GalNAc conjugates are hepatotropic and long-acting and have the potential to treat a wide range of diseases involving liver-expressed genes.

Custom Oligonucleotides Synthesis - KareBay Bio

We are an international provider of genomic services around the core business lines next generation sequencing, genotyping, gene expression, custom DNA sequencing, oligonucleotides, siRNA and gene synthesis.

Design and in-house synthesis of overlapping oligonucleotides to support the optimal gene construction method.

Chemical synthesis of oligonucleotides

The researchers design the oligonucleotides so that each piece needed for a particular gene has the same “barcode,” or identifying stretch of gene sequence.

They then use microbeads tagged with a sequence complementary to that barcode to pull those oligonucleotides out of an oligonucleotide pool.

GeneArt Gene Synthesis and Services

We have obtained a total of with hybridization unit sizes ranging from 16 to 22 oligonucleotides and Tm ranging from 62.5 to 69.6°C.

These oligonucleotides have been assembled into a full sized gene using either LCR or assembly PCR, followed by a PCR amplification ().

We call

The Chemical Synthesis of Oligonucleotides

These oligonucleotides can be assembled together by using either Ligase Chain Reaction (LCR) or assembly PCR.

We have processed the with Gene2Oligo using a hybridization unit size of 20 nucleotides and the software optimized Tm option.