oligonucleotide; gene synthesis; nucleic acids; synthetic biology
Oligonucleotide synthesis is the chemical synthesis of relatively ..
Microarray synthesis of DNA oligonucleotides costs a fraction of a penny per base. But such methods can’t make pieces longer than about 200 bases. And stitching those oligonucleotide fragments into gene-length sequences typically must be done one strand at a time, losing the multiplex advantage of the array synthesis.
Artificial gene synthesis - Wikipedia
And stitching those oligonucleotide fragments into gene-length sequences typically must be done one strand at a time, losing the multiplex advantage of the array synthesis.
custom oligo synthesis, oligonucleotides ..
2004 Jul 1;32(Web Server issue):W176-80
Gene2Oligo is a program that divides a long sequence into a set of contiguous oligonucleotides corresponding to both DNA strands.
Antisense Oligonucleotides - Integrated DNA …
Conjugation of small interfering RNA (siRNA) to an asialoglycoprotein receptor ligand derived from -acetylgalactosamine (GalNAc) facilitates targeted delivery of the siRNA to hepatocytes and . The ligands derived from GalNAc are compatible with solid-phase oligonucleotide synthesis and deprotection conditions, with synthesis yields comparable to those of standard oligonucleotides. Subcutaneous (SC) administration of siRNA–GalNAc conjugates resulted in robust RNAi-mediated gene silencing in liver. Refinement of the siRNA chemistry achieved a 5-fold improvement in efficacy over the parent design with a median effective dose (ED50) of 1 mg/kg following a single dose. This enabled the SC administration of siRNA–GalNAc conjugates at therapeutically relevant doses and, importantly, at dose volumes of ≤1 mL. Chronic weekly dosing resulted in sustained dose-dependent gene silencing for over 9 months with no adverse effects in rodents. The optimally chemically modified siRNA–GalNAc conjugates are hepatotropic and long-acting and have the potential to treat a wide range of diseases involving liver-expressed genes.
Custom Oligonucleotides Synthesis - KareBay Bio
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