First Strand cDNA Synthesis Kit for RT-PCR

Two step Reverse Transcriptase-Polymerase Chain Reaction (RT- PCR): Using ACCESS Quick RT-PCR SYSTEM (RT-PCR kit). Cat. No #A1702 (Promega) FOR First Strand cDNA Synthesis.

First Strand cDNA Synthesis Kit for RT-PCR ( AMV ) - …

followed by the First Strand cDNA Synthesis Kit for RT-PCR (AMV ..

for RT-PCR: First Strand cDNA PLUS Synthesis Kit: MMLV Reverse ..

Description
Using the 1st Strand cDNA Synthesis Kit, RNA is reversibly transcribed into single-stranded cDNA (1). In this method, AMV Reverse Transcriptase synthesizes the new cDNA strand at a site(s) determined by the type of primer used: at the 3'-end of the poly(A)-mRNA when Oligo-p(dT) is used as a primer, at nonspecific spots along the RNA template when using the Random Primer p(dN) 6 , or at a primer-binding site for a sequence-specific primer. The resulting first strand cDNA can then be used as a template for PCR.

AMV Reverse Transcriptase is well suited for the preparation of cDNA for use as a PCR template. Fully active in PCR buffer, AMV Reverse Transcriptase features a high specific activity, ensuring large amounts of template. At 42ºC, this highly processive enzyme is more efficient than M-MuLV Reverse Transcriptase.

First‐strand cDNA synthesis kit for RT‐PCR (AMV; ..

Hi,

This is the first time that I will be peforming qRT-PCR.

I will be carrying out a two-step qRT-PCR, which involve the first step of first strand cDNA synthesis.

I come across two kit which is the "Transcriptor First Strand cDNA Synthesis Kit" and "1st Strand cDNA Synthesis Kit for RT-PCR
(AMV)". Both o the kit are from Roche.

Can anyone help me with the choice between these two kits?

Thanks.

Reference:
Transcriptor First Strand cDNA Synthesis Kit -
1st Strand cDNA Synthesis Kit for RT-PCR (AMV) -

23/06/2014 · First Strand cDNA Synthesis kit for RT-PCR (AMV) ..
First Strand cDNA Synthesis Kit for RT PCR (AMV) ..

AMV First Strand cDNA Synthesis Kit | NEB

The PrimeScript First Strand cDNA Synthesis Kit contains all of the reagents necessary for synthesis of first-strand cDNA from total or poly(A)+ RNA using PrimeScript Reverse Transcriptase. Choose this cDNA synthesis kit when you want to synthesize full-length cDNA with provided primers (select from oligo dT or random 6-mer primers, both included in the kit.) This cDNA synthesis kit is powered by PrimeScript Reverse Transcriptase, which has exceptionally strong strand displacement activity and efficiently synthesizes cDNA. PrimeScript RTase is robust, versatile and well-suited for applications requiring full-length cDNA such as cDNA library construction. This PrimeScript-based cDNA synthesis kit also works well with other cDNA-dependent techniques including RT-PCR, cDNA probe preparation and real-time quantitative RT-PCR (qRT-PCR or RT-qPCR). The PrimeScript First Strand cDNA Synthesis Kit is ideal for reverse transcription of many different RNA templates, including GC-rich templates and RNAs with high levels of secondary structure.

The AMV First Strand cDNA Synthesis Kit combines AMV Enzyme Mix and ..

quantitative analysis by standard PCR or real-time PCR.

"ProtoScript II First Strand cDNA Synthesis Kit features two optimized mixes, ..

Invitrogen SuperScript First Strand Synthesis System for RT …