First Strand cDNA Synthesis Kit for RT-PCR ( AMV ) - …
for RT-PCR: First Strand cDNA PLUS Synthesis Kit: MMLV Reverse ..
Using the 1st Strand cDNA Synthesis Kit, RNA is reversibly transcribed into single-stranded cDNA (1). In this method, AMV Reverse Transcriptase synthesizes the new cDNA strand at a site(s) determined by the type of primer used: at the 3'-end of the poly(A)-mRNA when Oligo-p(dT) is used as a primer, at nonspecific spots along the RNA template when using the Random Primer p(dN) 6 , or at a primer-binding site for a sequence-specific primer. The resulting first strand cDNA can then be used as a template for PCR.
AMV Reverse Transcriptase is well suited for the preparation of cDNA for use as a PCR template. Fully active in PCR buffer, AMV Reverse Transcriptase features a high specific activity, ensuring large amounts of template. At 42ºC, this highly processive enzyme is more efficient than M-MuLV Reverse Transcriptase.
First‐strand cDNA synthesis kit for RT‐PCR (AMV; ..
This is the first time that I will be peforming qRT-PCR.
I will be carrying out a two-step qRT-PCR, which involve the first step of first strand cDNA synthesis.
I come across two kit which is the "Transcriptor First Strand cDNA Synthesis Kit" and "1st Strand cDNA Synthesis Kit for RT-PCR
(AMV)". Both o the kit are from Roche.
Can anyone help me with the choice between these two kits?
Transcriptor First Strand cDNA Synthesis Kit -
1st Strand cDNA Synthesis Kit for RT-PCR (AMV) -