An example of orthogonality in convergent synthesis
Some relevant examples are the synthesis of
An example of orthogonality in convergent synthesis is the combination of t-Boc/Bzl and Fmoc/tBu using supports whose linkage to the peptide can be either labile to acid, base, palladium or photolabile.
Useful methods in enzymatic synthesis of peptides: a
Some typical examples of this methodology are the synthesis of phospholipase A (124 residues with 14 cysteine residues and 7 disulfide bridges), the synthesis of the microprotein S (
Enzymatic Peptide Synthesis: from the Substrate Point …
Despite these drawbacks, solid-phase synthesis has many advantages over the classical system in solution: the reaction can be automated and the problem of solubilization of the peptide no longer exists since it remains attached to the solid matrix.
New developments in enzymatic peptide synthesis - …
Some relevant examples are the synthesis of the leading non-caloric sweetener aspartame, the lysine sweet peptide, kyotorphin, angiotensin, enkephalin and dynorphin (;
Enzymatic peptide synthesis in low water content …
When Merrifield introduced the method of solid-phase synthesis in 1963, the scientific community reacted with skepticism: the synthesis in solution was at that time well established and in the new proposed system the purification of the peptide could only be done after cleavage, with the concomitant cleavage of most of the byproducts accumulated during the synthesis (
protecting group in chemical and enzymatic peptide synthesis; ..
In biphasic systems, the partition of the peptide products from the aqueous phase that contains the enzyme to the organic phase drives the equilibrium towards synthesis, with the additional benefit that the product is no longer subjected to hydrolysis.