synthesis essay Deoxythymidylate

In a reaction mixture containing calf thymus DNA polymerase alpha (DNA nucleotidyltransferase; deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase; EC 2.7.7.7), calf thymus DNA unwinding protein, DNA, deoxyadenosine 5'-triphosphate and deoxythymidine 5'-triphosphate, a copolymer of deoxyadenylate and deoxythymidylate is synthesized after a lag period of 1-2 hr. In the presence of the four deoxyribonucleoside triphosphates only deoxyadenylate and deoxythymidylate are incorporated into the polymer and the rate of synthesis is decreased. The reaction variably occurs in the absence of DNA or DNA unwinding protein but with a greatly entended lag period. The optimal Mg2+ concentration for synthesis of the polymer of deoxyadenylate and deoxythymidylate is 1 mM, in contrast to an optimal Mg2+ concentration of 8 mM for DNA synthesis with activated DNA as template. Characterization of the product of de novo synthesis indicates that it is the alternating copolymer, poly(dA-dT).

Deoxyribonucleotides and Deoxythymidylate Synthesis ..

INHIBITION OF DNA SYNTHESIS by blocking BOTH purine and deoxythymidylate synthesis

disrupting deoxythymidylate synthesis

One mechanism of action of proguanil hydrochloride, via its metabolite cycloguanil, is inhibition of dihydrofolatereductase, which disrupts deoxythymidylate synthesis.

Deoxythymidylate synthesis essay

AB - Absorption, fluorescence and circular dichroism measurements on 33458 Hoechst deoxyribonucleic acid (DNA) complexes are consistent with the existence of two types of dye binding interactions. One type, which persists at elevated solution ionic strength, is highly specific for adenine thymine rich DNA. Dye bound under this condition exhibits efficient fluorescence and strong optical activity. A less specific, largely electrostatic interaction is associated with less intense fluorescence and weaker optical activity. The fluorescence of 33258 Hoechst and several other bisbenzimidazole dyes is less when bound to poly(deoxyadenylate 5 bromodeoxyuridylate) than when bound to poly(deoxyadenylate deoxythymidylate). Quenching of 33258 Hoechst fluorescence can also be used to detect biosynthetic incorporation of 5 bromodeoxyuridine into the DNA of living cells. This property of 33258 Hoechst should allow fluorescence activated cell and chromosome sorting according to the extent of DNA synthesis, providing a bridge between biochemical and cytologic analyses of processes related to DNA replication.

Deoxyribonucleotides and Deoxythymidylate Synthesis
Inhibition of dihydrofolate reductase in the malaria parasite disrupts deoxythymidylate synthesis

Thymidylate synthase - Wikipedia

Witmer H. Synthesis of deoxythymidylate and the unusual deoxynucleotide in mature DNA of Bacillus subtilis bacteriophage SP10 occurs by postreplicational modification of 5-hydroxymethyldeoxyuridylate. J Virol. 1981 Aug; 39 (2):536–54...

Genes for the Synthesis of Deoxythymidylate Monophosphate in T-Cell Lymphoma-Inducing Herpesviruses of Nonhuman Primates

Deoxynucleotide Synthesis CMM 12:2:14 - YouTube

DEOXYTHYMIDYLATE SYNTHESIS: The thymine-containing precursor of DNA is dTMP, which is synthesized after conversion of …

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