Solid-phase oligonucleotide synthesis - ATDBio
Solid-phase oligonucleotide synthesis
Glen Research has been active in supplying fluorescent dyes for labelling oligonucleotides for close to 30 years. Indeed, fluorescent dyes are probably the most commonly used tags for modifying oligonucleotides since they offer such sensitive detection in a wide variety of techniques varying from sequencing to genetic analysis. We offer a broad range of fluorophores covering most of the fluorescence spectrum. However we have never had a good substitute for Texas Red® or Alexa Fluor® 594, both of which are not amenable to the conditions of oligonucleotide synthesis and deprotection. Â
Oligonucleotide synthesis - dnareplicationsystem - …
Fully protected CPG-immobilized monomer, dimer and trimer oligonucleotides were used to study depurination during the chemical synthesis of oligonucleotides. Disappearance of the oligonucleotide during acid exposure time relative to an internal thymidine standard not subject to depurination was monitored by reverse phase HPLC analysis. Depurination half-times obtained for dichloroacetic acid (DCA) and trichloroacetic acid (TCA) in methylene chloride were found to be 3% DCA >> 15% DCA > 3% TCA. In order to understand the implications of depurination during DNA synthesis, the detritylation kinetics of model compounds DMT-dG-pT dimer and DMT-[17mer] mixed-base sequence were also measured. These results improve our ability to properly balance the contradictory goals of obtaining maximum detritylation with minimum depurination in oligonucleotide synthesis.
Oligonucleotide Synthesis | Chemical Compounds | …
Response of HEK-Blue™ hTLR9 cells to Class A CpG ODNs.
HEK-Blue™ hTLR9 cells, which express human TLR9 and an NF-κB-inducible SEAP reporter gene, were incubated in HEK-Blue Detection medium and stimulated with increasing concentrations of A-class CpG ODNs, ODN 1585, ODN 2216 or ODN 2236, and their corresponding GC control ODNs.
After 24h incubation, the levels of NF-ΚB-induced SEAP were determined by reading the OD at 655 nm.
Oligonucleotide Synthesizer - Technology Development …
Response of HEK-Blue™ mTLR9 cells to Class A CpG ODNs.
HEK-Blue™ mTLR9 cells, which express mouse TLR9 and an NF-κB-inducible SEAP reporter gene, were incubated in HEK-Blue Detection medium and stimulated with increasing concentrations of A-class CpG ODNs, ODN 1585, ODN 2216 or ODN 2236, and their corresponding GC control ODNs.
After 24h incubation, the levels of NF-kB-induced SEAP were determined by reading the OD at 655 nm.