Continuous enzymatic synthesis of biodiesel with Novozym 435

The biodiesel synthesis using a byproduct from palm oil refining and commercial immobilized lipases was demonstrated in this work. High fatty acids conversions were obtained in mild reaction conditions. Biodiesel yields higher than 80% were obtained in the reactions conducted with methanol or ethanol employing Novozym 435. The use of such a cheap feedstock and a biocatalyst to produce biodiesel, in addition to economic gains, represents an environment friendly process.

Continuous Enzymatic Synthesis of Biodiesel with Novozym ..

Biodiesel was also synthesized enzymatically with Novozym 435 in ..
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Continuous enzymatic synthesis of biodiesel with Novozym 435.

The enzymatic synthesis of biodiesel by a high-pressure semi-continuous process in near-critical carbon dioxide (NcCO2) was studied. Biodiesel synthesis was evaluated in both batch and semi-continuous systems to develop an effective process. Batch processing demonstrated the advantageous properties of NcCO2 as an alternative reaction medium. Three immobilized lipases (Novozym 435, Lipozyme RM IM, and Lipozyme TL IM from Novozymes) were tested, with Lipozyme TL IM the most effective, showing the highest conversion. Biodiesel conversion from several edible and non-edible oil feedstocks reached >92%. Higher conversion (99.0%) was obtained in a shorter time by employing repeated batch processes with optimized conditions: 44.3 g (500 mM) canola oil, a substrate molar ratio (methanol:oil) of 3:1, an enzyme loading of 20 wt% (of the oil used), at 30 °C, 100 bar, and 300 rpm agitation. The enzyme maintained 80.2% of its initial stability after being reused eight times. These results suggest that this method produces biodiesel energy-efficiently and environment-friendly.

the commercial immobilized lipase Novozym 435 was ..

This article describes the production of biodiesel from microalgae oil obtained by enzymatic catalysis. To preserve enzyme activity (Novozym 435 and Lipozyme TLIM) the methanol is reduced to a 1:1 oil molar ratio, working with high methyl acetate proportions. This is therefore the main methylating agent, shifting the chemical equilibrium towards biodiesel production. The synthesis was carried out with and without supercritical CO2 ; samples were analysed by 1H NMR and reversed phase HPLC. One of the main findings was the importance of using the two enzymes jointly to obtain the best activity. Biodiesel yield was also found to improve (80% in 24 hours) in direct relation to the excess of methylating agents in the 40-50ºC interval. Under supercritical conditions the dilution hardly affected lipase activity or, ipso facto, methyl ester productivity.

The continuous process for enzymatic synthesis of biodiesel using ..
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Investigation of the stability of Novozym ..

The biocatalysis of such esters by lipase-catalysis reactions under milder conditions has become a current industrial interest. Many reactions were conducted by immobilized lipase in a continuous packed-bed bioreactor for minimizing labors and overhead costs in the industry []. An optimized enzymatic catalysis of biodiesel production with higher yield at reduced cost in the optimal condition would be more appealing to the consumers and benefit to the manufacturers. The validity of the predicted model was examined by conducting experiments at the suggested optimum synthesis conditions. The value predicted by ridge max analysis was 83.31 ± 2.07%, and the actual value was 82.81 ± 0.98%.

Synthesis of Fatty Acid Ethyl Ester from ..

An optimal continuous production of biodiesel by methanolysis of soybean oil in a packed-bed reactor was developed using immobilized lipase (Novozym 435) as a catalyst in a tert-butanol solvent system. Response surface methodology (RSM) and Box-Behnken design were employed to evaluate the effects of reaction temperature, flow rate, and substrate molar ratio on the molar conversion of biodiesel. The results showed that flow rate and temperature have significant effects on the percentage of molar conversion. On the basis of ridge max analysis, the optimum conditions were as follows: flow rate 0.1mL/min, temperature 52.1°C, and substrate molar ratio 1:4. The predicted and experimental values of molar conversion were 83.31 ± 2.07% and 82.81 ± .98%, respectively. Furthermore, the continuous process over 30 days showed no appreciable decrease in the molar conversion. The paper demonstrates the applicability of using immobilized lipase and a packed-bed reactor for continuous biodiesel synthesis.

for the synthesis of biodiesel: Novozym 435 …

In this study, we developed a continuous packed-bed reactor, which was packed with Novozym 435 lipase, to produce biodiesel from soybean oil with methanol. tert-butanol was used as the solvent, because it is an ideal medium that enhances miscibility of methanol with vegetable oils as well as being regenerating agent of lipase [, , , ]. Statistical experiment design and RSM analysis were employed to investigate the affinities between the reaction variables (reaction temperature, flow rate, and substrate molar ratio) and response (molar conversion percentage), and to obtain the optimal conditions for continuous packed-bed reactor operation. This paper provides a feasible model for long-term operation of packed-bed reactor and sheds light on industrial scale production of biodiesel with enzymatic catalysts.