The role of ATP in the biosynthesis of coenzyme B12. | …
Biosynthesis of the corrin macrocycle of coenzyme B12 …
Isolation and characterization of intermediates of coenzyme B12 biosynthesis, a cobyrinic acid triamide, the a,c-diamide and their Co-(5′-deoxy-5′-adenosyl) derivatives, from Propionibacterium shermanii
The complete coenzyme B12 biosynthesis ..
Biochemical and genetic characterization of this motif confirms that the RNA directly binds coenzyme B12, and that it likely serves as a genetic control element for regulating expression of the 25-gene operon for cobalamin production in this pathogen.
two major coenzyme B 12-dependent enzyme families ..
Inadequate vitamin B-12 status during pregnancy and early childhood has been associated with adverse child health outcomes, including impaired cognitive development.
have the enzymes required for its biosynthesis.
Pathway 1 - Biosynthesis of nucleotides for incorporation into DNA and RNA;
Pathway 2 - Remethylation of homocysteine to form methionine (vitamin B12 serves as a coenzyme in this reaction);
3 - Methylation of substrates, including DNA, RNA, phospholipids, and proteins;
4 - MTHFR, which catalyzes the formation of 5-methylfolate needed for methylation reactions;
5 - Dihydrofolate reductase enzyme.
Comparative Genomics of the Vitamin B12 Metabolism …
Since is localized in the Golgi compartment, this enzyme might be a nonmitochondrial CoQ10 prenyltransferase. To test this, laboratories performed subcellular fractionation experiments on human ECs and detected only in Golgi compartments and not in mitochondria.
Studies about de novo CoQ10 synthesis in these subcellular fractions by incubating ECs with the hydroxy-4-benzoic acid- 13 C 6 precursor and observed a decrease in CoQ10- 13 C 6
production in Golgi compartments compared to mitochondrial fractions when expression is reduced.
These data indicate that is a CoQ10 biosynthetic enzyme located in the Golgi membrane compartment where large amounts of cellular CoQ10 are normally synthesized.