Chitin biosynthesis as a target for antifungals, pp.
The Danger of Chitin Synthesis Inhibitors - …
Nonomura and Ohara (1969) used pre-treatment such as dry heating, specialized growth media and long incubation times to isolate new species of Actinomadura, Microbispora, Micro tetraspora, Streptosporangium, Thermom- onospora and Thermoactinomyces. Many antibiotics are tested against a range of actinomycetes, bacteria and fungi representing types found in soil. From these, nystatin (50 mg/ml), acetidione (50 mg/ml), polymyxin B sulphate (5 mg/ml) and sodium penicillin (1 mg/ml) are generally selected for incorporation into a starch casein medium to achieve selective growth of actinomycetes on soil dilution plates.
to act as a chitin synthesis inhibitor
It was established that the final intermediate of chitin biosynthesis (UDP-N-acetylglucosamine) was formed in the isolated integuments in the presence of diflubenzuron and polyoxin-D.