(Chemical Synthesis Services ..
Synthesis Services (Seagoe Industrial Estate, Craigavon Armagh ..
Oligonucleotides were synthesized by phosphoramidite chemistry (Expedite 8900 synthesizer, standard protecting groups, standard deprotection) or purchased from CSS Chemical Synthesis Services, Craigavon and IBA, Göttingen. Phosphoramidites were from Proligo, Cruachem and ChemGenes. T7 transcription reactions were performed as described (). α-Thio-modified nucleoside triphosphate analogues were from Glen Research or IBA. Incorporation of hexa-ethylene glycol (HEG)-tethered anthracene was performed either chemically by using the respective phosphoramidite, or by transcription initiation (). Oligonucleotides were purified by HPLC and/or PAGE. Oligonucleotides and conjugates were 32P-labeled at their 3′-end with T4 RNA ligase or Klenow fragment of DNA Polymerase I, or 5′-end labeled with polynucleotide kinase, according to standard procedures (). The 11-mer anthracene conjugates were always synthesized chemically, while RNA 18-mers and 24-mers were either chemically synthesized, or prepared by run-off transcription.
obtained from CSS Chemical Synthesis Services, (Craigavon…
“Tom’s considerable expertise and experience within biocatalysis and chemical synthesis has been crucial in developing the Sciences, and more recently, Arran businesses. In addition, his ability to form incredibly strong relationships with our clients has been critical to the growth and success of these particular service offerings. I am delighted to announce Tom’s promotion to VP and wish him every success in his new role.”