Schematic outline of Mint cDNA synthesis.

Because cDNA synthesis is susceptible to interruption by secondary structures in the template RNA, the 5' ends of genes are typically underrepresented in cDNAs synthesized by conventional methods. During SMART cDNA synthesis truncated products resulting from premature termination of the reverse transcription reaction do not incorporate the SMART(er) oligonucleotide and consequently are not amplified during PCR. Thus, cDNA created using our SMART technology and amplified by long-distance PCR is enriched for full-length cDNA. Furthermore, because the 5’ SMART(er) sequence and modified oligo dT primer are not added onto genomic DNA or cDNA transcribed from ribosomal RNA, cDNA that is generated using SMART is free of these contaminating agents.

High Efficient cDNA synthesis kit with gDNA remover

Cultured cell lysis & cDNA Synthesis kit for real-time PCR

cDNA Synthesis & RT-PCR | NEB

The kit contains all components required for cDNA reactions for use with conventional thermal cyclers and real-time PCR instruments. In addition, the 50-reaction pack size includes 10 control reactions.

Reverse Transcription (cDNA Synthesis) | NEB

The synthesis of DNA from an RNA template, via reverse transcription, produces complementary DNA (cDNA). Reverse transcriptases (RTs) use an RNA template and a short primer complementary to the 3' end of the RNA to direct the synthesis of the first strand cDNA, which can be used directly as a template for the Polymerase Chain Reaction (PCR). This combination of reverse transcription and PCR (RT-PCR) allows the detection of low abundance RNAs in a sample, and production of the corresponding cDNA, thereby facilitating the cloning of low copy genes. Alternatively, the first-strand cDNA can be made double-stranded using DNA Polymerase I and DNA Ligase. These reaction products can be used for direct cloning without amplification. In this case, RNase H activity, from either the RT or supplied exogenously, is required.

cDNA prepared with Mint-2 kit can be normalized using  (Cat# NK003) to decrease the prevalence of high abundant transcripts.

microScript microRNA cDNA Synthesis Kit ..

SMART cDNA Synthesis Technology ensures uninterrupted cDNA synthesis, creating cDNAs with well-represented 5’ end sequences. Since terminal transferase activity and the subsequent SMART switching process occur preferentially at the 5' ends of eukaryotic mRNAs, truncated products resulting from premature termination of the reverse transcription reaction generally do not incorporate the SMART(er) oligonucleotide, and consequently are not amplified during PCR. Thus, cDNA pools created using our SMART technology and amplified by long-distance PCR are enriched for full-length cDNA. Additionally, because the 5’ SMART(er) sequence and modified oligo dT primer are not added onto genomic DNA or cDNA transcribed from ribosomal RNA, cDNA that is generated using SMART is free of these contaminating agents.

- for cDNA synthesis with gene-specific primers, or RT-PCR applications requiring fragments 500 bases

CDNA Synthesis Kits | Life Science Research | Bio-Rad

1. It depends on how much starting material you have and the primers/reverse transcriptase that you use. But basically smears are normal. Even when the rRNA is not visible.

2. I have never compared both, but I presume both would also be smears on the gel, as both would still produce varying lengths of cDNA.

3. The smear is fine. Just use the cDNA in a PCR to check, or run a spectro.

4. I would say it depends how many templates the primers stick to. The primer annealing step of 70C for 5 min prior to addition of reverse transcriptase decides how many templates have primers. Since there are no cyclic temperatures, the MMLV can only continue producing cDNAs from mRNAs (at 37C or 42C) that have primers annealed. So basically it's one RNA one cDNA.

Clontech offers cDNA Library Construction Kits that utilize the SMART cDNA synthesis method to generate full-length cDNA.

Evrogen Mint-2 cDNA synthesis kit: Method overview

Mint-2 cDNA synthesis kit is designed to synthesize full-length-enriched double stranded (ds) cDNA from total or poly(A)+ RNA. Synthesized cDNA can be used for construction of cDNA libraries, subtractive hybridization (SSH), high-throughput sequencing on the next generation sequencing platforms (Roche/454, ABI/SOLiD or Illumina/Solexa), and other applications.

The iScript™ cDNA synthesis kit performs across a broad range of concentrations

Encontre Cdna Synthesis. Mais de 1.000 resultados na web.

In-Fusion Cloning makes it easy to clone your SMARTer cDNA library into the pSMART2IF or pSMART2IFD linearized vectors (included in In-Fusion SMARTer kits) in just one 30 min reaction. Most importantly, since In-Fusion Cloning is designed to join fragments of DNA with 15 complementary bp at their ends, In-Fusion kits can be used to precisely transfer your SMARTer cDNA into ANY linearized vector. If you would like to clone your library into your own expression vector for functional analysis, simply amplify your vector by inverse PCR using primers that create linear vector ends that are complementary to the ends of the SMARTer cDNA. Primers must have two characteristics: the 5’ end of the primer must contain 15 bases that are complementary to 15 bases at one end of the DNA fragment to which the vector will be joined (i.e., the insert), and the 3’ end of the primer must contain sequence that is specific to the target vector.