High Efficient Reverse Transcription Kit for Real-time PCR

ReverTra Ace-α-® is an efficient and convenient kit to synthesize high quality cDNA. This kit contains the highly efficient reverse transcriptase "ReverTra Ace®", as well as other components optimized for the synthesis of long cDNAs suitable for RT-PCR. ReverTra Ace® is an M-MLV reverse transcriptase that has been improved by point mutation technology. ReverTra Ace® has two mutations in the polymerase and RNase H domains.

ReverTra Ace® qPCR RT Kit & Master Mix

NEW:  Tests for RNA Quality and Inhibitors of RT-PCR Analyses, optional
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High Efficient cDNA synthesis kit with gDNA remover

1. A Poly-A Polymerase adds a poly-A tail to the miRNA sequence.
2. A primer, containing oligo(dT) and a universal PCR primer sequence, anneals to the poly-adenylated RNA.
3. A Reverse Transcriptase synthesizes and appends to the primer DNA sequence complementary to the miRNA.
4. The final first strand cDNA product contains the miRNA complement sequence, a poly(dT) tract, and the universal PCR primer sequence. It is immediately ready for a real-time PCR assay containing a miRNA-specific and the universal PCR primer.

ReverTra Ace® qPCR RTMaster Mix withgDNA Remover

Control:
A built-in miRNA External RNA Control (ERC) helps test for inhibitors of reverse transcription. Control elements on the RT² miRNA PCR Arrays detect template synthesized from the ERC. A reproducible threshold cycle value from this control across your samples indicates a consistently high level of RNA quality and transcription efficiency giving you a greater degree of confidence in your results.

NEW:  From the experts in isolating RNA free of genomic DNA for real-time RT-PCR
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cDNA Synthesis For Real-Time PCR | Biocompare …

The kit contains all components required for cDNA reactions for use with conventional thermal cyclers and real-time PCR instruments. In addition, the 50-reaction pack size includes 10 control reactions.

cDNA Synthesis ; One-Step RT-PCR

The synthesis of DNA from an RNA template, via reverse transcription, produces complementary DNA (cDNA). Reverse transcriptases (RTs) use an RNA template and a short primer complementary to the 3' end of the RNA to direct the synthesis of the first strand cDNA, which can be used directly as a template for the Polymerase Chain Reaction (PCR). This combination of reverse transcription and PCR (RT-PCR) allows the detection of low abundance RNAs in a sample, and production of the corresponding cDNA, thereby facilitating the cloning of low copy genes. Alternatively, the first-strand cDNA can be made double-stranded using DNA Polymerase I and DNA Ligase. These reaction products can be used for direct cloning without amplification. In this case, RNase H activity, from either the RT or supplied exogenously, is required.

iScript cDNA Synthesis Kit | | Bio-Rad

One of the greatest advantages of SMART technology is its increased efficiency compared to traditional technologies such as adaptor ligation. Its high efficiency and sensitivity enables you to use a very limited quantity of starting material, such as microdissected tissues, laser-captured cells, biopsy samples, etc. As little as 1-2 ng of total RNA is sufficient for generating a highly representative cDNA pool for different downstream applications.