Cap rna synthesis | tecontthemjakedutucafoncpetab
Capped RNA Synthesis (E2040) | NEB
Similar to fungi, several protozoan parasites such as Encephalitozoan cuniculi, Plasmodium falciparum, and Trypanosoma species encode capping machinery in three separate polypeptide chains. In these organisms, the RNA triphosphatase appears similar to the metal-dependent Cet1 triphosphtase. In Leishmania, capping enzymes are synthesized in two parts with the Cet1-like RNA triphosphatase activity encoded in a single polypeptide, whereas the RNA guanylyltransferase and guanine-N7 methyltransferase activities are encoded in a bifunctional polypeptide ().
RNA modifications (synthesis of 5 prime cap) - YouTube
While activities required for cap 0 synthesis are conserved, the way in which the enzymes are organized differs across eukaryal and viral evolution. In fungi, the three enzymatic activities are encoded by separate genes, one for the metal-dependent triphosphatase, one for the guanylyltransferase, and one for the guanine-N7 methyltransferase ()., In metazoa and plants, capping activities are encoded in two polypeptides, one for the guanine-N7 methyltransferase and one that contains a bifunctional enzyme with an N-terminal metal-independent RNA triphosphatase domain fused to a C-terminal RNA guanylyltransferase domain, ().