(2011) Biosynthesis of Gold Nanoparticles: A Review.
TABLE 1: BIOLOGICAL AGENTS USED FOR GOLD NANOPARTICLES BIOSYNTHESIS
For particle size analysis of gold nanoparticles, the gold nanoparticle-fungus reaction solution was filtered with Whatman filter paper followed by filtration using 0.2μ filter to remove any spores or mycelia. Filtrate collected was dried, scraped with scalpel, and suspended in ethanol. Particle size was determined using Particle Size Analyzer (MALVERN Instruments) at K. C. Patel R&D center, CHARUSAT, Changa, India.
Biosynthesis of gold nanoparticles using ..
Gold nanoparticles (AuNPs) are a widespread research tool because of their oxidation resistance, biocompatibility and stability. Chemical methods for AuNP synthesis often produce toxic residues that raise environmental concern. On the other hand, the biological synthesis of AuNPs in viable microorganisms and their cell-free extracts is an environmentally friendly and low-cost process. In general, fungi tolerate higher metal concentrations than bacteria and secrete abundant extracellular redox proteins to reduce soluble metal ions to their insoluble form and eventually to nanocrystals. Fungi harbour untapped biological diversity and may provide novel metal reductases for metal detoxification and bioreduction. A thorough understanding of the biosynthetic mechanism of AuNPs in fungi is needed to reduce the time of biosynthesis and to scale up the AuNP production process. In this review, we describe the known mechanisms for AuNP biosynthesis in viable fungi and fungal protein extracts and discuss the most suitable bioreactors for industrial AuNP biosynthesis.