Biodiesel Synthesis and Evaluation: An Organic …

Recent environmental regulations regarding diesel fuel standards and the role the Midwest may play in future fuel production provides an opportunity to relate principles of organic chemistry to everyday life. Biodiesel is the transesterification product of triglycerides of vegetable oils with methanol to form fatty acid methyl esters (FAMEs). The resultant FAME fuel has decreased viscosity compared to vegetable oil and fuel properties similar to petroleum-based diesel fuel. In this two week lab sequence students synthesize and evaluate fatty acid methyl esters from simulated waste vegetable oil. To maximize product, students determine weight percent of free fatty acid in the sample and convert the acids to FAMEs via acid-catalyzed Fischer esterification. After conversion of FFA, the remaining mono-, di-, and triglycerides in the waste vegetable oil are converted to FAMEs by base-catalyzed transesterification. Finally, the completeness of the conversion product is determined indirectly by analysis of total, free, and combined glycerol in the final product by stoichiometric oxidation of vicinal alcohols by periodic acid. By determining the weight of glycerol in a specific weight sample of product biodiesel, the student determines whether the glycerol concentration is low enough to meet the specifications for marketable biodiesel.

BIODIESEL SYNTHESIS lab report - Scribd

Biodiesel Synthesis | Biodiesel | Diesel Fuel

Synthesis of Biodiesel from Waste Cooking Oil - sciepub

In the present study biodiesel was synthesized from Waste Cook Oil (WCO) by three-step method and regressive analyzes of the process was done. The raw oil, containing 1.9wt% Free Fatty Acid (FFA) and viscosity was 47.6mm2/s. WCO was collected from local restaurant of Sylhet city in Bangladesh. Transesterification method gives lower yield than three-step method. In the three-step method, the first step is saponification of the oil followed by acidification to produce FFA and finally esterification of FFA to produce biodiesel. In the saponification reaction, various reaction parameters such as oil to sodium hydroxide molar ratio and reaction time were optimized and the oil to NaOH molar ratio was 1:2, In the esterification reaction, the reaction parameters such as methanol to FFA molar ratio, catalyst concentration and reaction temperature were optimized. Silica gel was used during esterification reaction to adsorb water produced in the reaction. Hence the reaction rate was increased and finally the FFA was reduced to 0.52wt%. A factorial design was studied for esterification reaction based on yield of biodiesel. Finally various properties of biodiesel such as FFA, viscosity, specific gravity, cetane index, pour point, flash point etc. were measured and compared with biodiesel and petro-diesel standard. The reaction yield was 79%.

Biodiesel Synthesis: Use of Activated Carbon as …

This project aims to genetically engineer E. coli bacteria to significantly increase production of fatty acids, or biodiesel, from a biological matter feedstock. This potentially low-carbon fuel could replace fossil hydrocarbons currently used in on-road vehicles and aircraft. The major research goals include increasing the activity of key control enzymes that regulate the production of fatty acids, engineering biodiesel biosynthesis so it does not lead to concomitant accumulation of waste glycerol, and synthesis of tailored fuels that have desirable properties for a range of applications.

Enzymatic Biodiesel Synthesis Using a Byproduct …

The red portion of the structure represents the glycerol backbone of the fat, while the long portions are the fatty acids that serve as a source of carboxylic acids in the preparation of biodiesel. Biodiesel synthesis breaks the bonds between the glycerol and fatty acids and adds a methyl group (one carbon atom bonded to three hydrogen atoms, —CH3) to the end of the fatty acid.

The other reaction product is glycerol, which can be used in soapmaking. One of the three biodiesel molecules formed from the canola oil molecule above would be:

Biodiesel is synthesised by transesterification

Lesson 1
Properties of Various Cooking Oils
Lesson 2
Synthesis of
Lesson 3
Analysis of Biodiesel
Synthesis and Analysis of Biodiesel
The objective of this is to synthesis biodiesel and then to seperate and purify the biodiesel.
Background on Biodiesel
-It is a form of diesel fuel manufactured from vegetable oils, animal fats, or recycled restaurant greases
-It is made through a chemical process called Transeterification
-Biodiesel can be used as a pure fuel or blended with petroleum in any percentage
-It is important because its biodegradable, non-toxic, safer to handle
By : Melissa, Elliot, and Hannah
Chemistry Honors Period 5

-This is a process in which the ester groups of alcohol and the ester group of the vegetable oil trade places.
Structure of Biodiesel


This project seeks to engineer the most well understood organism in biology, Escherichia coli, as a microbial factory for fatty acid production. To achieve this goal, the investigator is taking a multi-pronged approach that addresses the problem at three different levels. First, the carbon flux to fatty acid biosynthesis will be increased by using genes from other organisms, which in turn should increase the specific productivity of fatty acids via the fermentation process. Second, different types of fatty acid analogs will be evaluated as potential fuel sources. The choices of compounds will be based on meeting or exceeding existing fuel criteria, while at the same time improving productivity and/or recoverability of product from the fermentation broth. Finally, by decoupling fatty acid analogue biosynthesis from triglyceride biosynthesis, it will be possible to produce biodiesel in microorganisms without the concomitant accumulation of glycerol waste.