Enzymatic synthesis of a CCK-8 tripeptide fragment in organic media.

Useful methods in enzymatic synthesis of peptides: a comparative study focusing on kinetically controlled synthesis of Ac-Phe-Leu-NH2 catalyzed by α-chymotrypsin.

Properties and synthetic applications of enzymes in organic solvents.

A novel synthesis of rasagiline via a chemoenzymatic dynamic kinetic resolution.

Enzymatic synthesis of Leu- and Met-enkephalin.

Additionally, similar to the incorporation of transition-metal complexes within supramolecular host-guest complexes, the use of artificial metalloenzymes enables the catalytic activity of transition-metal catalysts in biological environments, allowing synthetic catalysts to work collaboratively with other enzymes.

Enzymatic synthesis of aspartame.

This method sets the stage for the generation of artificial enzymes from innumerable combinations of PIX—proteins scaffolds and unnatural metal cofactors for various abiological transformations.

Design and engineering of artificial oxygen-activating metalloenzymes.

Ronald Breslow is Professor of Chemistry at Columbia University

Use of protected substrates in oligopeptide synthesis has led to a loss of continuity in the reaction sequence and a reduction in yield and productivity, limiting the usefulness of enzymes for such purpose.

Artificial gene synthesis - Wikipedia

Protease CLEAs are promising for peptide synthesis even though autoproteolysis can play a role in this case because of the close proximity and flexibility of the enzyme molecules.

mismatch binding enzymes of the mutS family ..

A 425 fold increase in enzyme activity was observed by the addition of 18-crown-6 to chymotrypsin in the synthesis of a dipeptide in acetonitrile medium (

Artificial Enzymes | Sigma-Aldrich

An interesting approach was designed for the enzymatic synthesis of the cholescystokinin C-terminal octapeptide (CCK-8), which is a biologically active peptide of therapeutic value as an agent for the control of gastrointestinal function (

Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids.

Design and synthesis of artificial enzymes - ScienceDirect

Several approaches have been implemented to improve the one-pot operation of chemocatalysts and biocatalysts, including partitioning in a biphasic system, incorporation into a supramolecular cage, catalysts compartmentation, and development of artificial metalloenzymes [,].

Improving the catalytic performance of an artificial metalloenzyme by computational design.

BioSynthesis: Artificial Enzymes

With the aid of computational, molecular and structural biology, functional artificial metalloenzymes have been developed by de novo design or protein redesign processes [].

Directed evolution of artificial metalloenzymes for in vivo metathesis.

A novel approach to the rational design of artificial enzymes

In this sense, enzymatic synthesis of peptides is a less mature technology than chemical synthesis and no general protocols of synthesis are available, being each situation a particular case that has to be extensively studied and optimized to be technologically competitive.