Ambion kit: RNA synthesis x µl DEPC treated water
What are microRNA (miRNA) mimics? – Bio-Synthesis, …
Total RNA, including miRNAs, was polyadenylated andreverse-transcribed with a poly(T) adapter into cDNAs for real-time PCRusing the miRNA-specific forward primer and the sequence complementaryto the poly(T) adapter as the reverse primer.
In Vitro mRNA Synthesis - Heald Lab - Google Sites
To evaluate possiblepre-amplificationbias, we compared the expression of 384 miRNAs in three differentcancer cell lines with Megaplex RT, with or without an additionalpre-amplificationstep.
mMESSAGE mMACHINE™ T7 Transcription Kit
Our convenient and high-quality real-time RT-PCR accessory products complete the RT² Profiler™ PCR Array System and the RT² PCR Primer Assays. The other components needed for real-time PCR analysis are SYBR Green PCR master mix, and an optional RNA QC array.
Custom Long RNA Transcription Synthesis Services - …
Real-time RT-PCR-specific errors in the quantification of microRNAtranscripts are easily compounded with any variation in the amount ofstarting material between the samples, e.g.
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The single molecule fluorescent hybridization (smFISH) method differs from conventional approaches by using many short (about 20 base pairs long) oligonucleotide probes to target different regions of the same mRNA transcript (; ). Each oligo is conjugated with only one fluorophore and thus faintly visible by itself. Binding of multiple oligos to the same transcript yields a bright spot, indicative of a single mRNA transcript. Since mis-bound probes are unlikely to co-localize, this method effectively reduces false-positive signal from non-specific probe binding. The small oligo size allows the probes to efficiently penetrate through target tissue, yielding robust detection of even low-abundant transcripts. Subsequently, the total number of fluorescent spots within a single cell or region can be unambiguously counted and compared across different developmental stages and genetic backgrounds.