Design, synthesis, and activity of 2,3 …

Patients over 1 month of age with arterial oxygen pressures of less than 60 mm Hg were found to have elevated red cell 2,3-diphosphoglycerate (2,3-DPG) levels and blood with a decreased affinity for oxygen. The increase in 2,3-DPG was proportional to the degree of hypoxemia. In patients under 1 month of age this relationship was not observed. Red cells from adults, but not newborns, showed rapid increases in 2,3-DPG when incubated under nitrogen. Adult, but not fetal, deoxyhemoglobin was shown to facilitate in vitro synthesis of 2,3-DPG by binding this organic phosphate and relieving the product inhibition of 2,3-DPG mutase.

2 3 dpg synthesis essay - Kursy Komputerowe

2,3-Diphosphoglycerate synthesis by human white …

as 2,3-diphosphoglycerate (2,3-DPG ..

Where the O tension is low, as in the venous blood, the Bohr effect and 2,3-DPG combine to reduce the affinity of Hgb for O , releasing O to tissues.

2,3-diphosphoglycerate (2,3-DPG) ..

Incubation of adult and fetal red blood cells in a medium containing high concentrations of inorganic phosphate showed a different response in the synthesis of 2,3 diphosphoglycerate. This was increased only in fetal red cells, while an evident increase in fructose 1,6 diphosphate was seen in both adult and fetal red blood cells.

ATP synthesis from PEP, 2-PG, 3-PG and 2,3-DPG with ultracentrifugation fractions

, 2,3-DPG), presumably to try to ..

Throughout a wide range change in oxygen affinity as measured by the P50 is linear with respect to the 2,3-DPG concentration; a change of 430 mμmoles of 2,3-DPG/ml of red blood corpuscles (RBC) resulting in a change of the P50 of 1 mm Hg. It appears that the 2,3-DPG of the adult's red cells responds rapidly to metabolic and environmental influences and in turn effects metabolism and the cellular environment. Many of these effects are not shared by the red cells of the newborn.

The increase in 2,3-DPG was proportional to ..

With deoxygenation the a1ß2 interface tightens lessening the affinity of Hgb for oxygen. This conformation is stabilized by proton binding and 2,3-DPG.

2,3-Dpg 2,3-diphosphonooxypropanoic acid

Many cases with vascular dementia have multiple lacunaes and diffuse demyelination in the white matter (vascular leukoencephalopathy; VLE). One of the major causes of VLE is chronic hypoxia. 2, 3-diphosphoglycerate (DPG) in red cells plays an important role in oxygen delivery in microcirculation. It decreases the oxygen affinity of hemoglobin and facilitates the unloading of oxygen from hemoglobin, displacing the oxygen dissociation curve to the right. In this study red cell DPG and P50 (PO2 at half saturation) in demented patients with VLE were compared with those in non-demanted, post-stroke patients (NDPS) who had localized brain lesions and in control cases. The results were as follows. 1) DPG was inversely proportional to hemoglobin concentration. DPG levels were significantly elevated in NDPS patients compared with controls, but was not different between VLE patients and controls. 2) P50 was inversely proportional to hemoglobin concentration. P50 was significantly reduced in VLE patients, but was not different between NDPS patients and controls 3) P50 was proportional to DPG levels. VLE patients had significantly lower P50 than controls, while P50 was different between NDPS and controls. The results suggest that DPG and P50 are compensatorily elevated in non-demented post-ischemic patients, while such compensation is lacking in demented patients with vascular leukoencephalopathy. In such demented patients the red cell oxygen affinity which causes tissue hypoxia has a tendeny to increase above the normal level. Decompensation of red cell DPG synthesis may have an important implication in the pathogenesis of vascular leukoencephalopathy.